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Invitrogen
Progesterone Receptor Monoclonal Antibody (PR-AT 4.14)
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FIGURE: 1 / 24
Progesterone Receptor Antibody (MA1-410) in IHC (P)
Immunohistochemical analysis of Progesterone Receptor was performed using formalin-fixed paraffin-embedded human breast cancer (ER/PR/Her2 +ve) tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber a... View More
Product Details
MA1-410
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
Published species
Bovine,
Dog,
Guinea pig,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Sheep
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
PR-AT 4.14
Immunogen
Synthetic peptide corresponding to residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-410 detects the progesterone receptor (PR) from human, non-human primate, bovine, rat, rabbit, and mouse tissues. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor.
MA1-410 has been successfully used in Western blot, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from T47D whole cell lysate.
MA1-410 performs better by western blot using BSA as the blocking agent and in antibody diluent.
The MA1-410 immunogen is a synthetic peptide corresponding to the binding domain residues G(533) L P Q V Y P P Y L N Y L R P(547) of human Progesterone Receptor.
MA1-410 can be used with blocking peptide PEP-016.
Target Information
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor; progesterone-receptor; steroid hormone receptor; unnamed protein product
Gene Aliases: 9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B
UniProt ID: (Rabbit) P06186, (Human) P06401, (Rat) Q63449, (Mouse) Q00175
Entrez Gene ID: (Rabbit) 100009094, (Bovine) 100848466, (Human) 5241, (Rat) 25154, (Mouse) 18667
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
receptor binding
steroid binding
protein binding
zinc ion binding
lipid binding
enzyme binding
estrogen response element binding
identical protein binding
sequence-specific DNA binding
metal ion binding
ATPase binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
hormone binding
ovulation from ovarian follicle
luteinization
epithelial cell maturation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of gene expression
positive regulation of smooth muscle cell migration
diterpenoid metabolic process
intracellular receptor signaling pathway
mammary gland development
response to progesterone
response to cocaine
negative regulation of apoptotic process
steroid hormone mediated signaling pathway
response to estrogen
estrous cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of epithelial cell proliferation
progesterone receptor signaling pathway
female mating behavior
tertiary branching involved in mammary gland duct morphogenesis
cellular response to gonadotropin stimulus
cellular response to follicle-stimulating hormone stimulus
cellular response to luteinizing hormone stimulus
glandular epithelial cell maturation
signal transduction
cell-cell signaling
positive regulation of gene expression
intracellular steroid hormone receptor signaling pathway
paracrine signaling
lung alveolus development
maintenance of protein location in nucleus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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