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Agrisera
PsbO2/33 kDa of the oxygen evolving complex (OEC) of PSII Polyclonal Antibody
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FIGURE: 1 / 1
PsbO2/33 kDa of the oxygen evolving complex (OEC) of PSII Antibody (AS14-2825) in WB
30 µg of thylakoid protein from Arabidopsis thaliana Columbia-0 extracted with 330 m M sorbitol, 50 m M Hepes-KOH (p H 7.8) were separated on 15% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 5% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 for 6 h at 4°C with agitation. The antibody solution was ... View More
Product Details
AS14-2825
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide chosen from Arabidopsis thaliana PsbO2.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
PSBO2 encodes a nuclear-encoded, chloroplast-targeted extrinsic subunit of photosystem II that accumulates on the luminal side of the thylakoid membrane as part of the oxygen-evolving complex. The gene produces an mRNA that is translated as a precursor polypeptide with an N-terminal chloroplast transit peptide that is removed upon import, yielding a soluble, predominantly beta-strand-rich lumenal protein of the conserved PsbO/manganese-stabilizing protein family that associates non-covalently with the PSII core rather than spanning the membrane. Functionally, PsbO2 contributes to efficient oxygen evolution by stabilizing the water-oxidizing Mn4CaO5 catalytic cluster and helping maintain the proper ionic and structural environment required for water splitting; in Arabidopsis, genetic analyses indicate that PsbO2 is the minor isoform relative to PsbO1 yet is important for PSII performance under light stress and has a specific role in regulating phosphorylation state and turnover/repair of the PSII reaction-center D1 protein, consistent with a contribution to PSII maintenance in addition to catalysis support.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT3G50820; F18B3.100; MSP-2; OEC33; PSBO; PSBO-2
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