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Invitrogen
Pyruvate Carboxylase Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Pyruvate Carboxylase Antibody (PA5-60552) in WB
Western blot analysis of Pyruvate Carboxylase in human cell lines HeLa and U-251MG using a Pyruvate Carboxylase Polyclonal Antibody (Product # PA5-60552). Corresponding PC RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Product Details
PA5-60552
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Pyruvate Carboxylase
Conjugate
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2646175
Product Specific Information
Immunogen sequence: GADVVDVAAD SMSGMTSQPS MGALVACTRG TPLDTEVPME RVFDYSEYWE GARGLYAAFD CTATMKSGNS DVYENEIPGG QYTNLHFQ
Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.
Target Information
Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 6.4.1.1; PCB; PYC; Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase
Gene Aliases: PC; PCB
UniProt ID: (Human) P11498
Entrez Gene ID: (Human) 5091
Molecular Function:
ligase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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