Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Pig (97%), Chicken (84%), Rhesus Monkey (100%), Bovine (96%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: PCB; pyruvate carboxylase; Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase
Gene Aliases: PC; PCB; Pcx
Molecular Function: ligase