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Invitrogen
Pyruvate Carboxylase Polyclonal Antibody
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FIGURE: 1 / 4
Pyruvate Carboxylase Antibody (PA5-78100) in ICC/IF
PCB antibody detects PCB protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: PCB protein stained by PCB antibody (Product # PA5-78100) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-78100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Pyruvate Carboxylase. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.69 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Pig (97%), Chicken (84%), Rhesus Monkey (100%), Bovine (96%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 6.4.1.1; PCB; PYC; Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase
Gene Aliases: PC; PCB; Pcx
UniProt ID: (Human) P11498, (Rat) P52873
Entrez Gene ID: (Human) 5091, (Rat) 25104
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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