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Agrisera
RA/Rubisco activase Polyclonal Antibody
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FIGURE: 1 / 2
RA/Rubisco activase Antibody (AS22-4868) in WB
Samples from left to right: 1 – MW marker 2 – 10 µg of Arabidopsis thaliana whole leaf extract 3 – 10 µg of Hordeum vulgare whole leaf extract 4 – 10 µg Chlamydomonas reinhardtii whole cell extract 10 µg/well of respective cell extract was denatured with Invitrogen LDS sample buffer (4 X) at 70°C/5 min. Samples were separated on Invitrogen Nu PAGE Bis-Tris 4-12% SDS-PAGE 45 min and blotted for 1 h to Invitrogen PVDF (pore size of 0.45 um), using: wet transfer. Blot was blocked with 5% milk in TBS-T for: 1 h/RT with agitation. Blot was incu... View More
Product Details
AS22-4868
Product Specifications
Species Reactivity
Algae,
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Purified, recombinant Rubisco activase from Gossypium hirsutum.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare
Target Information
Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA. There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize,Chlamydomonas). Alpha is about 46-47 kDa, beta is about 42 kDa. Species that have only one form have the beta form.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT2G39730; AT2G39730.1; RA; RCA; Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic; RUBISCO ACTIVASE; T5I7.18
Gene Aliases: AT2G39730; RCA; RUBISCO ACTIVASE; T5I7.18; T5I7.3
UniProt ID: (Arabidopsis) P10896
Entrez Gene ID: (Arabidopsis) 818558
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