Proteintech

RAB3B Polyclonal Antibody

View all (6) RAB3B antibodies

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Questions & Answers

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RAB3B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15774-1-AP ( RAB3B Antibody) at dilution of 1:50 (under 40x lens).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15774-1-AP ( RAB3B Antibody) at dilution of 1:50 (under 10x lens).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 40x lens).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 10x lens).

RAB3B Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Western Blot (WB) — U-251 cells were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug RAB3B antibody (15774-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

15774-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

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Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAB3B Fusion Protein Ag8441 (1-219 aa encoded by BC005035)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MASVTDGKT GVKDASDQNF DYMFKLLIIG NSSVGKTSFL FRYADDTFTP AFVSTVGIDF KVKTVYRHEK RVKLQIWDTA GQERYRTITT AYYRGAMGFI LMYDITNEES FNAVQDWATQ IKTYSWDNAQ VILVGNKCDM EEERVVPTEK GQLLAEQLGF DFFEASAKEN ISVRQAFERL VDAICDKMSD SLDTDPSMLG SSKNTRLSDT PPLLQQNCSC (1-219 aa encoded by BC005035)

Target Information

Protein transport. Probably involved in vesicular traffic. Target protein may play a role in secretory granule function and exocytosis in pancreatic acinar cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B.

Cell death & disease

Hu J,Wang Q,Fan X,Zhen J,Wang C,Chen H,Liu Y,Zhou P,Zhang T,Huang T,Wang R,Lv Z

Wed Feb 15 00:00:00 EST 2023

Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B.

Cell death & disease

Hu J,Wang Q,Fan X,Zhen J,Wang C,Chen H,Liu Y,Zhou P,Zhang T,Huang T,Wang R,Lv Z

Wed Feb 15 00:00:00 EST 2023

Human-Specific Transcriptome of Ventral and Dorsal Midbrain Dopamine Neurons.

Annals of neurology

Monzón-Sandoval J,Poggiolini I,Ilmer T,Wade-Martins R,Webber C,Parkkinen L

Mon Jun 01 00:00:00 EDT 2020

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Human molecular genetics

Smith GA,Rocha EM,McLean JR,Hayes MA,Izen SC,Isacson O,Hallett PJ

Mon Sep 01 00:00:00 EDT 2014

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Human molecular genetics

Smith GA,Rocha EM,McLean JR,Hayes MA,Izen SC,Isacson O,Hallett PJ

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: brain antigen RAB3B; Ras-related protein Rab-3B

Gene Aliases: 2610528C18Rik; RAB3B

UniProt ID: (Human) P20337, (Mouse) Q9CZT8

Entrez Gene ID: (Human) 5865, (Mouse) 69908

transport intracellular protein transport vesicle docking involved in exocytosis small GTPase mediated signal transduction protein secretion protein transport synaptic vesicle exocytosis regulation of exocytosis peptidyl-cysteine methylation antigen processing and presentation Rab protein signal transduction positive regulation of dopamine uptake involved in synaptic transmission regulation of vesicle size

Process(es)

GTPase activity protein binding GTP binding GDP binding myosin V binding nucleotide binding