Proteintech

RAB3B Polyclonal Antibody

View all (6) RAB3B antibodies

Questions & Answers

Questions & Answers

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RAB3B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15774-1-AP ( RAB3B Antibody) at dilution of 1:50 (under 40x lens).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15774-1-AP ( RAB3B Antibody) at dilution of 1:50 (under 10x lens).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 40x lens).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 10x lens).

RAB3B Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Western Blot (WB) — U-251 cells were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug RAB3B antibody (15774-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

15774-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB3B Fusion Protein Ag8441 (1-219 aa encoded by BC005035) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.18 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MASVTDGKT GVKDASDQNF DYMFKLLIIG NSSVGKTSFL FRYADDTFTP AFVSTVGIDF KVKTVYRHEK RVKLQIWDTA GQERYRTITT AYYRGAMGFI LMYDITNEES FNAVQDWATQ IKTYSWDNAQ VILVGNKCDM EEERVVPTEK GQLLAEQLGF DFFEASAKEN ISVRQAFERL VDAICDKMSD SLDTDPSMLG SSKNTRLSDT PPLLQQNCSC (1-219 aa encoded by BC005035)

Target Information

Protein transport. Probably involved in vesicular traffic. Target protein may play a role in secretory granule function and exocytosis in pancreatic acinar cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.

Human molecular genetics

Smith GA,Rocha EM,McLean JR,Hayes MA,Izen SC,Isacson O,Hallett PJ

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: brain antigen RAB3B; Ras-related protein Rab-3B

Gene Aliases: 2610528C18Rik; RAB3B

UniProt ID: (Human) P20337, (Mouse) Q9CZT8, (Rat) Q63941

Entrez Gene ID: (Human) 5865, (Mouse) 69908, (Rat) 81755

transport intracellular protein transport vesicle docking involved in exocytosis small GTPase mediated signal transduction protein secretion protein transport synaptic vesicle exocytosis regulation of exocytosis peptidyl-cysteine methylation antigen processing and presentation Rab protein signal transduction positive regulation of dopamine uptake involved in synaptic transmission regulation of vesicle size protein localization to plasma membrane

Process(es)

GTPase activity protein binding GTP binding GDP binding myosin V binding nucleotide binding