Proteintech

RAB3B Polyclonal Antibody

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RAB3B Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAB3B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 15774-1-AP ( RAB3B Antibody) at dilution of 1:50 (under 40x lens).

RAB3B Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RAB3B Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug RAB3B antibody (15774-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Western Blot (WB)

1:500-1:1000

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RAB3B Fusion Protein Ag8441

Conjugate

Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Protein transport. Probably involved in vesicular traffic. Target protein may play a role in secretory granule function and exocytosis in pancreatic acinar cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: brain antigen RAB3B; Ras-related protein Rab-3B

Gene Aliases: 2610528C18Rik; RAB3B

UniProt ID: (Human) P20337, (Mouse) Q9CZT8, (Rat) Q63941

Entrez Gene ID: (Human) 5865, (Mouse) 69908, (Rat) 81755

Function(s)

small GTPase mediated signal transduction protein transport regulation of exocytosis peptidyl-cysteine methylation antigen processing and presentation positive regulation of dopamine uptake involved in synaptic transmission regulation of vesicle size transport intracellular protein transport vesicle docking involved in exocytosis protein secretion synaptic vesicle exocytosis Rab protein signal transduction protein localization to plasma membrane

Process(es)

GTPase activity protein binding GTP binding GDP binding myosin V binding nucleotide binding

RAB3B Polyclonal Antibody

Product Details

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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