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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • RAC1/RAC2/RAC3 Antibodies

          Invitrogen

          RAC1/RAC2/RAC3 Polyclonal Antibody

          1 Published Figure
          2 References
          View all (5) RAC1/RAC2/RAC3 antibodies

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          Cite RAC1/RAC2/RAC3 Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (1)
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          RAC1/RAC2/RAC3 Antibody (PA5-17519) in WB

          Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), Jurkat (Lane 3), U-87 MG (Lane 4), 3T3-L1 (Lane 5) and NIH/3T3 (Lane 6). The blot was probed with Anti-RAC1/RAC2/RAC2 Polyclonal Antibody (Product # PA5-17519, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 20 kDa band corresponding to RAC1/RAC2/RAC3 was observed across the cell lin... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          RAC1/RAC2/RAC3 Polyclonal Antibody

          Product Details

          PA5-17519

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Non-human primate, Rat, Xenopus

          Published species

          C. elegans, Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues surrounding Thr138 of human Rac1
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          39 µg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          Contains

          no preservative

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10986181

          Product Specific Information

          It is not recommended to aliquot this antibody.

          This antibody is not cross-reactive with other small GTPases.

          Target Information

          The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cell migration-inducing gene 5 protein; EN-7 protein; GX; p21-Rac1; p21-Rac2; p21-Rac3; Protein EN-7; Ras-like protein TC25; RAS-related C3 botulinum substrate 2; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) L homeolog; Ras-related C3 botulinum toxin substrate 2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); Ras-related C3 botulinum toxin substrate 3; ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3); Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac1; rho family, small GTP binding protein Rac3; Small G protein

          View more View less

          Gene Aliases: AI323801; AI452260; AL023026; D5Ertd559e; EN-7; Gx; HSPC022; MIG5; p21-Rac1; p21-Rac2; Rac; Rac-1; RAC1; Rac1B; RAC2; RAC3; TC-25; TC25; XELAEV_18045321mg; Xrac

          View more View less

          UniProt ID: (Bovine) P62998, (Human) P63000, (Human) P15153, (Bovine) Q9TU25, (Human) P60763, (Mouse) P63001, (Rat) Q6RUV5, (Mouse) Q05144, (Mouse) P60764

          View more View less

          Entrez Gene ID: (Bovine) 281440, (Human) 5879, (Human) 5880, (Bovine) 327671, (Human) 5881, (Bovine) 619066, (Mouse) 19353, (Rat) 363875, (Mouse) 19354, (Rat) 366957, (Mouse) 170758, (Rat) 688319, (Xenopus) 734382

          View more View less

          Function(s)
          protein binding GTP binding Rho GDP-dissociation inhibitor binding GTPase activity Rab GTPase binding enzyme binding protein kinase binding GTP-dependent protein binding thioesterase binding histone deacetylase binding protein kinase regulator activity calcium-dependent protein binding nucleotide binding immunoglobulin small GTPase
          Process(es)
          neuron migration positive regulation of protein phosphorylation regulation of cell migration cell motility semaphorin-plexin signaling pathway auditory receptor cell morphogenesis mast cell chemotaxis epithelial cell morphogenesis movement of cell or subcellular component inflammatory response hyperosmotic response cell adhesion cell-matrix adhesion G-protein coupled receptor signaling pathway small GTPase mediated signal transduction axon guidance blood coagulation cell proliferation regulation of cell size response to wounding anatomical structure morphogenesis regulation of hydrogen peroxide metabolic process positive regulation of lamellipodium assembly regulation of fibroblast migration positive regulation of cell-substrate adhesion regulation of neuron maturation cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development lamellipodium assembly actin cytoskeleton organization actin filament polymerization platelet activation positive regulation of actin filament polymerization T cell costimulation ruffle organization negative regulation of interleukin-23 production substrate adhesion-dependent cell spreading positive regulation of Rho protein signal transduction intracellular signal transduction Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of apoptotic process positive regulation of phosphatidylinositol 3-kinase activity engulfment of apoptotic cell cell-cell junction organization bone resorption positive regulation of DNA replication vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway negative regulation of receptor-mediated endocytosis anatomical structure arrangement dendrite morphogenesis homeostasis of number of cells within a tissue regulation of defense response to virus by virus regulation of small GTPase mediated signal transduction positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly synaptic transmission, GABAergic Wnt signaling pathway, planar cell polarity pathway regulation of respiratory burst cellular response to mechanical stimulus dopaminergic neuron differentiation protein localization to plasma membrane positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly positive regulation of substrate adhesion-dependent cell spreading chemotaxis actin filament organization signal transduction positive regulation of cell proliferation regulation of cell-substrate adhesion cell projection assembly regulation of T cell proliferation regulation of mast cell degranulation regulation of protein kinase activity regulation of mast cell chemotaxis lymphocyte aggregation regulation of neutrophil migration positive regulation of protein targeting to mitochondrion neuron projection development positive regulation of cell adhesion mediated by integrin neuromuscular process controlling balance endocytosis phagocytosis, engulfment cytoskeleton organization metabolic process dendrite development cell migration regulation of cell morphogenesis Rac protein signal transduction neuromuscular process
          It has to be done as per old AB suggested Products section.
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