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Western blot of RAD1/RRAD was performed using A) HEK293 lysates overexpressing several HA-tagged mouse GTPases (including Rrad) and B) 100ug of WT and KO lysates of mouse heart. Lysates were probed with an HA-specific antibody (A) and a RAD1/RRAD polyclonal antibody (Product# PA5-37885) (B) at a dilution of 0.5ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-HRRSMPVDERDLQ, from the internal region of RRAD (aa36-48)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human, rat, dog, pig and cow based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
GTP-binding protein RAD; RAD; RAD and GEM like GTP binding 3; RAS; ras associated with diabetes; Ras-like GTP-binding protein rad; Ras-related associated with diabetes; REM3