|Tested species reactivity||Bacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant RecA expressed in E. coli.|
|Storage buffer||PBS with 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4924 detects RecA in E. coli samples.
PA1-4924 has been successfully used in Western blot, ELISA, and immunoprecipitation procedures. By western blot the antibody detects a ~38 kDa protein (RecA) in E. coli samples.
PA1-4924 immunogen corresponds to recombinant RecA protein.
E. coli RecA protein plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its co-protease activity after DNA damage, and induces many proteins related to DNA repair, including RecA itself. Since RecA has many functions and interacts with various proteins in the cell, the protein complexes can be detected by immunoprecipitaion with the anti-RecA antibody. RecA forms foci on nucleoids in the cells after exposure to DNA damaging treatments. The foci is visualized by immuno-staining of the cells using this antibody as the first antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.