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Invitrogen
recA Polyclonal Antibody
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FIGURE: 1 / 2
recA Antibody (PA1-4924) in WB
Western blot analysis of recA protein in crude extract of E. coli using recA Polyclonal Antibody (Product # PA1-4924). Lane 1, Purified recA 8 ng. Lane 2, Wild type. Lane 3, ΔrecA mutant. Lane 4, ΔlexA mutant (SOS genes overexpressed). Antibody used at 1:3,000 dilution. Cell extracts, 2 µg.
Product Details
PA1-4924
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Highly purified full-length recombinant E. coli protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-4924 detects RecA in E. coli samples.
PA1-4924 has been successfully used in Western blot, ELISA, and immunoprecipitation procedures. By western blot the antibody detects a ~38 kDa protein (RecA) in E. coli samples.
PA1-4924 immunogen corresponds to recombinant RecA protein.
Target Information
E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Expression is induced by SOS response caused by DNA damage. The RecA protein has multiple functions, such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loops and Holliday structures during homologous recombination, and co-protease activities that promote self-cleavage of the LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its is homologous to the eukaryotic Rad51 protein and Dmcl protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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