Invitrogen

RAG1 Polyclonal Antibody

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Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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RAG1 Antibody in Western Blot (WB) — Western blot analysis of RAG1 in whole cell extract using RAG1 polyclonal antibody (Product # PA5-85711) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Product Details

PA5-85711

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RAG1.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.85 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2792850

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (84%), Pig (90%), Rabbit (96%), Chicken (86%), Rhesus Monkey (98%).

Positive Control: Molt-4.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase RAG1; Endonuclease RAG1; MGC43321; RAG-1; RAG-1 Antibody; recombination activating gene 1 Antibody; recombination activating protein 1; RING finger protein 74; RING-type E3 ubiquitin transferase RAG1; RNF74 Antibody; V(D)J recombination-activating protein 1

Gene Aliases: RAG-1; RAG1; RNF74

UniProt ID: (Human) P15918

Entrez Gene ID: (Human) 5896

Molecular Function: DNA metabolism protein endodeoxyribonuclease nucleic acid metabolism protein

Function(s)

adaptive immune response pre-B cell allelic exclusion DNA recombination immune response histone monoubiquitination B cell differentiation T cell differentiation in thymus V(D)J recombination T cell homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of T cell differentiation thymus development protein autoubiquitination negative regulation of thymocyte apoptotic process nucleic acid phosphodiester bond hydrolysis

Process(es)

DNA binding endonuclease activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity histone binding protein homodimerization activity sequence-specific DNA binding metal ion binding ubiquitin protein ligase activity