Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue . To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE (PA1-075) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1-2 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-075 detects receptor for advanced glycation endproducts (RAGE) from mouse and rat samples.
PA1-075 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.
The PA1-075 immunogen is a synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE. This sequence is 100% conserved in mouse and 70% conserved in humans. This peptide (Cat. # PEP-194) is available for use in neutralization and control experiments.
The receptor for advanced glycation endproducts (RAGE) is a member of the Immunoglobin superfamily of cell surface markers. This protein is able to interact with many molecules including: advanced glycation endproducts (AGE), amphoterin, and ligands. Research has shown that the accumulation of RAGE ligands in the biological system (endothelium mononuclear phagocytes, neurons and smooth muscle cells) can lead to destructive tissue diseases such as diabetic retinopathy, amyloidoses, tumors and inflammation disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amyloid-beta accumulation, neurogenesis, behavior, and the age of rats.
PA1-075 was used in immunohistochemistry - frozen section to study the relationship between brain biochemistry and behavior that are age-related
|Church RM,Miller MC,Freestone D,Chiu C,Osgood DP,Machan JT,Messier AA,Johanson CE,Silverberg GD||Behavioral neuroscience (128:523)||2014|
Blockage of receptor for advanced glycation end products prevents development of cardiac dysfunction in db/db type 2 diabetic mice.
PA1-075 was used in western blot to investigate the role of receptor for advanced glycation end products during development of cardiac dysfunction in db/db type 2 diabetic mice
|Nielsen JM,Kristiansen SB,Nørregaard R,Andersen CL,Denner L,Nielsen TT,Flyvbjerg A,Bøtker HE||European journal of heart failure (11:638)||2009|
High-mobility group box 1 protein is an inflammatory mediator in necrotizing enterocolitis: protective effect of the macrophage deactivator semapimod.
PA1-075 was used in western blot to study the role of high-mobility group box 1 protein in necrotizing enterocolitis
|Zamora R,Grishin A,Wong C,Boyle P,Wang J,Hackam D,Upperman JS,Tracey KJ,Ford HR||American journal of physiology. Gastrointestinal and liver physiology (289:G643)||2005|
Direct proteomic mapping of the lung microvascular endothelial cell surface in vivo and in cell culture.
PA1-075 was used in western blot to study the proteome of the rat lung microvascular endothelial cell surface in vivo and in cultured rat lung ECs
|Durr E,Yu J,Krasinska KM,Carver LA,Yates JR,Testa JE,Oh P,Schnitzer JE||Nature biotechnology (22:985)||2004|
Vitamin E increases S100B-mediated microglial activation in an S100B-overexpressing mouse model of pathological aging.
PA1-075 was used in immunohistochemistry to investigate the effect of vitamin E on S100B-mediated microglial activation
|Bialowas-McGoey LA,Lesicka A,Whitaker-Azmitia PM||Glia (56:1780)||2008|