Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 17
RAN Antibody (PA5-79913) in ICC/IF
Immunocytochemistry analysis of Ran using anti-Ran antibody (Product # PA5-79913) . Ran was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Ran antibody (Product # PA5-79913) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize... View More
Product Details
PA5-79913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Ran recombinant protein (Position: A2-L216).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747028
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PC-3 whole cell, human RT4 whole cell, human A549 whole cell, human CACO-2 whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse A20 whole cell. IHC: mouse small intestine tissue, rat small intestine tissue, human mammary cancer tissue, human intestinal cancer tissue, human lung cancer tissue. ICC/IF: U20S cell IP: HepG2 whole cell. Flow: A431 cell, U937 cell.
Target Information
Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Androgen receptor-associated protein 24; ARA25; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; OTTHUMP00000166078; OTTHUMP00000166102; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein
Gene Aliases: ARA24; Gsp1; OK/SW-cl.81; RAN; Rasl2-8; TC4
UniProt ID: (Human) Q9CWI7, (Rat) P62828, (Mouse) P62827
Entrez Gene ID: (Human) 5901, (Rat) 84509, (Mouse) 19384
chromatin binding
transcription coactivator activity
GTPase activity
protein binding
GTP binding
GDP binding
protein domain specific binding
protein complex binding
poly(A) RNA binding
dynein intermediate chain binding
androgen receptor binding
importin-alpha family protein binding
pre-miRNA binding
pre-miRNA transporter activity
cadherin binding involved in cell-cell adhesion
nucleotide binding
small GTPase
ribosomal large subunit export from nucleus
ribosomal small subunit export from nucleus
protein import into nucleus, translocation
DNA metabolic process
tRNA export from nucleus
protein export from nucleus
mitotic spindle organization
mitotic nuclear division
signal transduction
small GTPase mediated signal transduction
spermatid development
viral process
hippocampus development
actin cytoskeleton organization
androgen receptor signaling pathway
gene silencing by RNA
positive regulation of protein binding
cellular protein complex localization
pre-miRNA export from nucleus
positive regulation of transcription, DNA-templated
cell division
cellular response to mineralocorticoid stimulus
intracellular transport of virus
cell-cell adhesion
protein localization to nucleolus
microtubule cytoskeleton organization
protein import into nucleus
nucleocytoplasmic transport
metabolic process
cellular protein localization
ribosomal subunit export from nucleus
transport
intracellular protein transport
cell cycle
protein transport
regulation of protein binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support