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Invitrogen

RAN Polyclonal Antibody

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RAN Polyclonal Antibody

Product Details

PA5-79913

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

Immunoprecipitation (IP)

0.5-2 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human Ran recombinant protein (Position: A2-L216).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4

Contains

0.01mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2747028

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human PC-3 whole cell, human RT4 whole cell, human A549 whole cell, human CACO-2 whole cell, rat thymus tissue, rat PC-12 whole cell, mouse thymus tissue, mouse A20 whole cell. IHC: mouse small intestine tissue, rat small intestine tissue, human mammary cancer tissue, human intestinal cancer tissue, human lung cancer tissue. ICC/IF: U20S cell IP: HepG2 whole cell. Flow: A431 cell, U937 cell.

Target Information

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Androgen receptor-associated protein 24; ARA25; G protein; RAN/TC4; ARA24; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OTTHUMP00000166078; OTTHUMP00000166102; RAN; RanGTPase; ras-like protein; Ras-like protein TC4; Ras-related nuclear protein; unnamed protein product

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Gene Aliases: ARA24; Gsp1; OK/SW-cl.81; RAN; Rasl2-8; TC4

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UniProt ID: (Human) P62826, (Rat) P62828, (Mouse) P62827

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Entrez Gene ID: (Human) 5901, (Rat) 84509, (Mouse) 19384

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Function(s)
nucleotide binding magnesium ion binding chromatin binding RNA binding GTPase activity obsolete small monomeric GTPase activity nuclear export signal receptor activity protein binding GTP binding hydrolase activity GDP binding protein domain specific binding macromolecular complex binding cadherin binding dynein intermediate chain binding metal ion binding protein heterodimerization activity importin-alpha family protein binding pre-miRNA binding poly(A) RNA binding protein complex binding small GTPase
Process(es)
ribosomal subunit export from nucleus ribosomal large subunit export from nucleus ribosomal small subunit export from nucleus mitotic sister chromatid segregation mitotic cell cycle glycolytic process DNA metabolic process protein import into nucleus protein export from nucleus nucleocytoplasmic transport mitotic spindle organization spermatid development protein localization protein transport viral process hippocampus development actin cytoskeleton organization protein complex localization pre-miRNA export from nucleus positive regulation of protein import into nucleus GTP metabolic process cell division snRNA import into nucleus cellular response to mineralocorticoid stimulus protein localization to nucleolus microtubule cytoskeleton organization mitotic nuclear division small GTPase mediated signal transduction metabolic process positive regulation of protein binding cellular protein localization cellular protein complex localization protein import into nucleus, translocation transport intracellular protein transport cell cycle signal transduction regulation of protein binding
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