|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) A D L A N E E K P A L A(13) C of human RanBP3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-084 detects Ran binding protein 3 (RanBP3) from human and rat cells.
PA1-084 has been successfully used in Western blot and immunofluorescence procedures. By Western blot this antibody detects an ~60 kDa protein representing RanBP3 from HeLa cell extracts. Immunofluorescence staining of RanBP3 in NRK cells with PA1-084 results in diffuse nuclear staining.
The PA1-084 immunogen is a synthetic peptide corresponding to residues M(1) A D L A N E E K P A L A(13) C of human RanBP3. The PA1-084 immunizing peptide (Cat. # PEP-177) is available for use in neutralization and control experiments.
Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane.
RanBP3 functions as a cofactor for CRM1-mediated nuclear export and stimulates export by enhancing the affinity of CRM1 for RanGTP and substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection.
PA1-084 was used in western blot to study the roles of viral RNA synthesis and viral ribonucleoprotein nuclear export in the mechanism by which sphingosine kinase-1 exerts its pro-viral effects during influenza infection
|Seo YJ,Pritzl CJ,Vijayan M,Bomb K,McClain ME,Alexander S,Hahm B||PloS one (8:null)||2013|