Immunofluorescent analysis of RAP1 showing staining in the nucleus and cytoplasm of A375 cells. A375 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RAP1 polyclonal antibody (Product # PA5-27588) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 90 and 342 of Human RAP1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27588 targets RAP1 in IHC (P), IP, and WB applications and shows reactivity with Human and mouse samples.
The PA5-27588 immunogen is recombinant fragment corresponding to a region within amino acids 90 and 342 of Human RAP1.
The gene encodes a protein that is part of a complex involved in telomere length regulation. Pseudogenes are present on chromosomes 5 and 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.