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Invitrogen
RAP1GAP Polyclonal Antibody
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FIGURE: 1 / 1
RAP1GAP Antibody (PA5-87968) in WB
Western blot analysis of RAP1GAP in extracts of various cell lines. Samples were incubated with RAP1GAP Polyclonal antibody (Product # PA5-87968) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-87968
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 508-727 of human Rap1GAP (NP_0011391301)
Conjugate
Form
Liquid
Concentration
1.56 mg/mL
Amount
156 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: AMGLSNKKPN TVSTSHSGSF APNNPDLAKA AGISLIVPGK SPTRKKSGPF GSRRSSAIGI ENIQEVQEKR ESPPAGQKTP DSGHVSQEPK SENSSTQSSP EMPTTKNRAE TAAQRAEALK DFSRSSSSAS SFASVVEETE GVDGEDTGLE SVSSSGTPHK RDSFIYSTWL EDSVSTTSGG SSPGPSRSPH PDAGKLGDPA CPEIKIQLEA SEQHMPQLGC; Positive Samples: U-87MG, 293T, SGC-7901, LO2; Cellular Location: Golgi apparatus membrane, Peripheral membrane protein
Target Information
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Rap1 GTPase-activating protein 1; Rap1GAP
Gene Aliases: KIAA0474; RAP1GA1; RAP1GAP; RAP1GAP1; RAP1GAPII; RAPGAP
UniProt ID: (Human) P47736
Entrez Gene ID: (Human) 5909
adaptive immune response
phagocytosis
signal transduction
positive regulation of cell-cell adhesion
regulation of GTPase activity
positive regulation of GTPase activity
negative regulation of neuron differentiation
positive regulation of phagocytosis
regulation of small GTPase mediated signal transduction
establishment of localization in cell
cell-cell adhesion
negative regulation of microvillus assembly
negative regulation of thyroid gland epithelial cell proliferation
cellular response to glial cell derived neurotrophic factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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