|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide from near the carboxy terminus of human RAP80.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2.5 µg/ml|
|Western Blot (WB)||2 ug/ml|
A suggested positive control is 293 cell lysate.
PA5-20413 can be used with blocking peptide PEP-0530.
RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
BRCA1-A complex subunit RAP80; RAP80, X2HRIP110; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor interacting protein 110; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1
9430016E08Rik; D330018D10Rik; D630032M02Rik; RAP80; RIP110; RXRIP110; UIMC1; X2HRIP110