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Invitrogen
RAP80 Polyclonal Antibody
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FIGURE: 1 / 3
RAP80 Antibody (PA5-77181) in ICC/IF
Immunofluorescence analysis of RAP80 in U2OS cells. Samples were treated with UV-A laser (cell damage treatment) and incubated with RAP80 polyclonal antibody (Product # PA5-77181), followed by GFP fusion protein expression (green, DNA damage marker) and DAPI (nuclear staining).
Product Details
PA5-77181
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human RAP80.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRCA1-A complex subunit RAP80; Receptor-associated protein 80; retinoid X receptor interacting protein 110; Retinoid X receptor-interacting protein 110; RIP110; Ubiquitin interaction motif-containing protein 1; unnamed protein product
Gene Aliases: 9430016E08Rik; D330018D10Rik; D630032M02Rik; RAP80; RGD1307009; RIP110; RXRIP110; UIMC1; X2HRIP110
UniProt ID: (Human) Q96RL1, (Mouse) Q5U5Q9, (Rat) Q5PQK4
Entrez Gene ID: (Human) 51720, (Mouse) 20184, (Rat) 290997
DNA repair
regulation of DNA repair
double-strand break repair
chromatin organization
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
response to ionizing radiation
mitotic G2/M transition checkpoint
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
DNA repair-dependent chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
chromatin modification
G2 DNA damage checkpoint
histone H2A K63-linked deubiquitination
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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