Invitrogen

RARB Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

3 References

View all (10) RARB antibodies

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Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

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RARB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of RARB was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with RARB Polyclonal Antibody (Product # PA1-811) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27034, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents BeWo having no expression of RARB. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

RARB Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), Mouse Prostate (Lane 2) and MCF-7 (Lane3). The blots were probed with Anti-Retinoic Acid Receptor beta Rabbit Polyclonal Antibody (Product # PA1-811, 1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution).An isoform of 53kDa was observed in SH-SY5Y cell line, a 38kDa band was observed in Mouse Prostate tissue and around 50kDa band was observed in MCF-7 cell line tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

RARB Antibody in ChIP Assay (ChIP) — Published figure using RARB polyclonal antibody (Product # PA1-811) in ChIP Assay

RARB Antibody in Gel Shift (GS) — Published figure using RARB polyclonal antibody (Product # PA1-811) in Gel Shift

RARB Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in SH-SY5Y and HEK-293 and not seen in PANC-1, OVCAR-3 and BeWo using RARB Polyclonal Antibody (Product # PA1-811) in Western Blot.

Product Details

PA1-811

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100 - 1:250

ChIP assay (ChIP)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(429) S V S P S S V E N S G V S Q S P L L Q(448) of mouse RAR beta.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum diluted in PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2253602

Product Specific Information

PA1-811 detects retinoic acid receptor (RAR) beta from human and mouse tissues. This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma.

PA1-811 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~52 kDa protein representing RAR beta from SH-SY5Y (human neuroblastoma) cell extract.

The PA1-811 immunogen is a synthetic peptide corresponding to residues P(429) S V S P S S V E N S G V S Q S P L L Q(448) of mouse RAR beta. This immunizing peptide (Cat. # PEP-005) is available for use in neutralization and control experiments.

Target Information

Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Wnt8a and Wnt3a cooperate in the axial stem cell niche to promote mammalian body axis extension.

Developmental dynamics : an official publication of the American Association of Anatomists

Cunningham TJ,Kumar S,Yamaguchi TP,Duester G

PA1-811 was used in EMSA to discuss how Wnt8a and Wnt3a contribute to body axis extension

Mon Jun 01 00:00:00 EDT 2015

Retinoic acid controls body axis extension by directly repressing Fgf8 transcription.

Development (Cambridge, England)

Kumar S,Duester G

PA1-811 was used in ChIP assay and EMSA to investigate the role of retinoic acid in body axis extension and its mechanism

Fri Aug 01 00:00:00 EDT 2014

A role for Hsp90 in retinoid receptor signal transduction.

Molecular biology of the cell

Holley SJ,Yamamoto KR

PA1-811 was used in western blot to investigate the role of heat shock protein (Hsp90) in retinoid receptor signal transduction

Fri Dec 01 00:00:00 EST 1995

Bioinformatics

Protein Aliases: HBV-activated protein; hepatitis B virus activated protein; Nuclear receptor subfamily 1 group B member 2; RAR beta; RAR-b; RAR-beta; RAR-Beta2; RAR-Beta4; RAR-epsilon; retinoic acid; retinoic acid receptor; Retinoic acid receptor beta; retinoic acid receptor, beta polypeptide

Gene Aliases: A830025K23; HAP; MCOPS12; NR1B2; RARB; RARbeta1; RRB2

UniProt ID: (Human) P10826, (Mouse) P22605

Entrez Gene ID: (Human) 5915, (Mouse) 218772

Molecular Function: C4 zinc finger nuclear receptor