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RARB Antibody (MA1-811) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK-MSR (lane 1), A431 (lane 2), T47D (lane 3), NIH/3T3 (lane 4), MCF-7 (lane 5), L6 (lane 6) and IMR-32 (lane 7). The blots were probed with Anti-Retinoic Acid Receptor beta Mouse Monoclonal Antibody (Product # MA1-811, 1:500-1:2000 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 37 kDa corresponding to Retinoic Acid Receptor beta was observed across cell lines tested ... View More
Product Details
MA1-811
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Fish,
Mouse,
Non-human primate,
Yeast
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
R beta 28
Immunogen
Synthetic peptide corresponding to the C-terminal sequence of the mouse RAR beta.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2177762
Product Specific Information
MA1-811 detects retinoic acid receptor (RAR) beta from human and mouse tissues. This antibody cross-reacts slightly with RAR alpha when expressed in COS cells, though the level of signal on Western blots is very low relative to that seen with RAR beta. It does not detect RAR alpha or gamma from cell lines or tissue extract.
MA1-811 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~52 kDa protein in rat tissue extract representing RAR beta. Also, by Western blot MA1-811 detects an unidentified ~120 kDa protein which can be competed away with the immunizing peptide. This protein is most notable in human SH-SY5Y and COS cells. Due to the extremely low levels of RAR expressed in native tissues, it is recommended that sensitive detection systems, such as enhanced chemiluminescence, be used.
The MA1-811 immunogen is a synthetic peptide corresponding to the C-terminal sequence of mouse RAR beta.
Reconstitute with PBS.
MA1-811 can be used with blocking peptide PEP-005.
Target Information
Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HBV-activated protein; hepatitis B virus activated protein; Nuclear receptor subfamily 1 group B member 2; RAR beta; RAR-b; RAR-beta; RAR-Beta2; RAR-Beta4; RAR-epsilon; retinoic acid; retinoic acid receptor; Retinoic acid receptor beta; retinoic acid receptor, beta polypeptide
Gene Aliases: A830025K23; HAP; MCOPS12; NR1B2; RARB; RARbeta1; RRB2
UniProt ID: (Human) P10826, (Mouse) P22605
Entrez Gene ID: (Human) 5915, (Rat) 24706, (Mouse) 218772
Molecular Function:
C4 zinc finger nuclear receptor
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
glandular epithelial cell development
outflow tract septum morphogenesis
retinal pigment epithelium development
growth plate cartilage development
transcription initiation from RNA polymerase II promoter
signal transduction
positive regulation of cell proliferation
negative regulation of cell proliferation
striatum development
regulation of myelination
negative regulation of chondrocyte differentiation
embryonic hindlimb morphogenesis
multicellular organism growth
positive regulation of apoptotic process
negative regulation of apoptotic process
steroid hormone mediated signaling pathway
positive regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
embryonic eye morphogenesis
retinoic acid receptor signaling pathway
embryonic digestive tract development
ventricular cardiac muscle cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
neurogenesis
positive regulation of programmed cell death
retina development in camera-type eye
bone development
negative regulation of cartilage development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
RNA polymerase II regulatory region sequence-specific DNA binding
DNA binding
steroid hormone receptor activity
retinoic acid receptor activity
drug binding
zinc ion binding
protein complex binding
retinoid X receptor binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
metal ion binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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