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Invitrogen
RASGRF1 Polyclonal Antibody
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FIGURE: 1 / 1
RASGRF1 Antibody (PA5-78412) in WB
Western blot analysis of RASGRF1 in various tissue extracts (30 µg). Samples were incubated with RASGRF1 polyclonal antibody (Product # PA5-78412) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG. Separated by 5% SDS-PAGE. Competitors antibody diluted at 1:200.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-78412
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Rat RASGRF1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: mouse brain, rat brain
Predicted reactivity: Rat (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RASGRF1 is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. Studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASPP1; guanine nucleotide-releasing protein; KIAA0771; PPP1R13B; Ras guanine release factor beta; ras-specific nucleotide exchange factor CDC25; unnamed protein product
Gene Aliases: CDC25; CDC25Mm; GNRP; Grf1; Grfbeta; P140 RAS-GRF; p190; P190-A; p190RhoGEF; Ras-GRF1
UniProt ID: (Mouse) P27671, (Rat) P28818
Entrez Gene ID: (Mouse) 19417, (Rat) 192213
small GTPase mediated signal transduction
Ras protein signal transduction
cell proliferation
neuron projection development
response to endoplasmic reticulum stress
positive regulation of Rac protein signal transduction
intracellular signal transduction
positive regulation of MAPK cascade
positive regulation of GTPase activity
regulation of Ras protein signal transduction
positive regulation of Ras protein signal transduction
positive regulation of fibroblast proliferation
regulation of synaptic plasticity
regulation of neuronal synaptic plasticity
activation of GTPase activity
postsynaptic modulation of chemical synaptic transmission
regulation of N-methyl-D-aspartate selective glutamate receptor activity
response to amphetamine
cellular response to insulin stimulus
negative regulation of MAPK cascade
cellular response to antibiotic
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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