Proteintech

RB1CC1 Polyclonal Antibody

Product Image Gallery

RB1CC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using RB1CC1 antibody 17250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

RB1CC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

RB1CC1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RB1CC1 Antibody in Immunoprecipitation (IP) — IP result of anti-RB1CC1 (IP:17250-1-AP, 5 ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 1600 ug.

Product Details

17250-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 15 publications 15 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 7 publications 7 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RB1CC1 Fusion Protein Ag10508 (1241-1594 aa encoded by BC017556) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Immunogen sequence: AIQTALKEF KLEREVVEKE LLEKVKHLEN QIAKSPAIDS TRGDSSSLVA ELQEKLQEEK AKFLEQLEEQ EKRKNEEMQN VRTSLIAEQQ TNFNTVLTRE KMRKENIIND LSDKLKSTMQ QQERDKDLIE SLSEDRARLL EEKKKLEEEV SKLRSSSFVP SPYVATAPEL YGACAPELPG ESDRSAVETA DEGRVDSAME TSMMSVQENI HMLSEEKQRI MLLERTLQLK EEENKRLNQR LMSQSMSSVS SRHSEKIAIR DFQVGDLVLI ILDERHDNYV LFTVSPTLYF LHSESLPALD LKPGEGASGA SRRPWVLGKV MEKEYCQAKK AQNRFKVPLG TKFYRVKAVS WNKKV (1241-1594 aa encoded by BC017556 )

Target Information

The protein encoded by this gene interacts with signaling pathways to coordinately regulate cell growth, cell proliferation, apoptosis, autophagy, and cell migration. This tumor suppressor also enhances retinoblastoma 1 gene expression in cancer cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Autophagy

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Thu Oct 03 00:00:00 EDT 2019

Systematic analysis of ATG13 domain requirements for autophagy induction.

Autophagy

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Thu Oct 03 00:00:00 EDT 2019

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

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Fri Jul 05 00:00:00 EDT 2019

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Mon Nov 05 00:00:00 EST 2018

RB1CC1-enhanced autophagy facilitates PSCs activation and pancreatic fibrogenesis in chronic pancreatitis.

Cell death & disease

Li L,Wang G,Hu JS,Zhang GQ,Chen HZ,Yuan Y,Li YL,Lv XJ,Tian FY,Pan SH,Bai XW,Sun B

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Remodeling of ER-exit sites initiates a membrane supply pathway for autophagosome biogenesis.

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Fri Sep 01 00:00:00 EDT 2017

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Tue Aug 01 00:00:00 EDT 2017

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Jung J,Nayak A,Schaeffer V,Starzetz T,Kirsch AK,Müller S,Dikic I,Mittelbronn M,Behrends C

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Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium.

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The Journal of cell biology

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Oncotarget

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Imaging autophagy.

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Tue Jul 01 00:00:00 EDT 2014

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Fri Nov 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 200 kDa FAK family kinase-interacting protein; coiled coil forming protein 1; Coiled-coil-forming protein 1; FAK family kinase-interacting protein of 200 kDa; FIP200; LaXp180; phosphatase 1, regulatory subunit 131; RB1-inducible coiled-coil 1; RB1-inducible coiled-coil protein 1

Gene Aliases: 2900055E04Rik; 5930404L04Rik; ATG17; CC1; FIP200; KIAA0203; LaXp180; PPP1R131; RB1CC1; RBICC

UniProt ID: (Human) Q8TDY2, (Mouse) Q9ESK9

Entrez Gene ID: (Human) 9821, (Mouse) 12421, (Rat) 312927

Molecular Function: scaffold/adaptor protein