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Agrisera
RCD1/Radical cell death 1 Polyclonal Antibody
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FIGURE: 1 / 2
RCD1/Radical cell death 1 Antibody (AS12-2602) in WB
5 mg of total protein from Arabidopsis thaliana seedlings expressing RCD 1-HA under native promoter extracted with RIPA buffer for protein extraction (50 m M Tris-HCl p H 7,5,150 m M Na Cl, 1% Triton X-100,0.5% sodium deoxycholate, protease inhibitor cocktail 1:100 50 µm MG 132) were used for immunoprecipitation and afterwards separated on 10% SDS-PAGE and blotted 1 h to PVDF using tank transfer. Blots were... View More
Product Details
AS12-2602
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RCD1 protein sequence.
Conjugate
Form
Lyophilized
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
RCD1 encodes a nuclear protein that functions as an interaction hub integrating environmental and hormonal cues into transcriptional regulation. In Arabidopsis, RCD1 localizes predominantly to the nucleus and is built around an N-terminal WWE protein-protein interaction domain together with a central region resembling the core catalytic fold of poly(ADP-ribose) polymerases (PARP-like) and a conserved C-terminal region; consistent with this modular architecture, RCD1 binds numerous transcription factors from multiple families and engages many partners through short linear motifs within intrinsically disordered regions of those transcription factors. Functionally, genetic and physiological studies support that RCD1 modulates stress and development, including responses to reactive oxygen species and hormone signaling, and it can restrain specific NAC transcription factors (e.g., ANAC013/ANAC017) that mediate mitochondrial retrograde signaling, thereby coordinating chloroplast and mitochondrial redox-associated pathways and shaping the expression of stress-responsive nuclear genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARABIDOPSIS THALIANA P8 (INTERACTING PROTEIN); AT1G32230; ATP8; AtRCD1; CEO; CEO1; F3C3.1; F3C3_1; RADICAL-INDUCED CELL DEATH1; REDOX IMBALANCED 1; RIMB1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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