Invitrogen

REST Polyclonal Antibody

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REST Polyclonal Antibody

Product Details

PA5-78148

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human REST. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736515

Product Specific Information

Positive Control: HeLa, HeLa nuclear, U87-MG, U87-MG nuclear extract

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Neural-restrictive silencer factor; neuron restrictive silencer factor; neuron-restrictive silencer factor; RE1-silencing transcription factor; RE1-silencing transcription factor variant E1a/E2/E3/E4c; RE1-silencing transcription factor variant E1a/E2/E3/E5; RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i; RE1-silencing transcription factor variant E1a/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1a/E2/E4; RE1-silencing transcription factor variant E1a/E2/E5; RE1-silencing transcription factor variant E1a/E2a/E2k; RE1-silencing transcription factor variant E1a/E2d/E4g; RE1-silencing transcription factor variant E1a/E2e/E4h; RE1-silencing transcription factor variant E1a/E2f/E4e; RE1-silencing transcription factor variant E1a/E2k/E2i/E3/E4j; RE1-silencing transcription factor variant E1b/E2/E3/E5; RE1-silencing transcription factor variant E1b/E2/E3/N3b/E4i; RE1-silencing transcription factor variant E1b/E2/E3/N3c/E4; RE1-silencing transcription factor variant E1b/E2a/E2k; RE1-silencing transcription factor variant E1b/E2c/E2j/E3/E4; RE1-silencing transcription factor variant E1b/E2e/E4h; RE1-silencing transcription factor variant E1c/E2/E3/E5; RE1-silencing transcription factor variant E1c/E2a/E2k; RE1-silencing transcription factor variant E1c/E2g/E3/E4; repressor binding to the X2 box; X2 box repressor

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Gene Aliases: NRSF; REST; WT6; XBR

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UniProt ID: (Human) Q13127

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Entrez Gene ID: (Human) 5978

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Function(s)
core promoter sequence-specific DNA binding core promoter binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding outward rectifier potassium channel activity protein complex binding transcription regulatory region DNA binding metal ion binding C2H2 zinc finger transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter hematopoietic progenitor cell differentiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter negative regulation of cell proliferation negative regulation of gene expression negative regulation of aldosterone biosynthetic process cellular response to drug positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation by host of viral transcription negative regulation of neuron differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of calcium ion-dependent exocytosis negative regulation of insulin secretion negative regulation of neurogenesis cardiac muscle cell myoblast differentiation histone H4 deacetylation cellular response to electrical stimulus cellular response to glucocorticoid stimulus potassium ion transmembrane transport negative regulation of cortisol biosynthetic process negative regulation of dense core granule biogenesis negative regulation of mesenchymal stem cell differentiation negative regulation of amniotic stem cell differentiation
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