|Immunoprecipitation (IP)||1-4 µl|
|Western Blot (WB)||1:500-1:5000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Syn. Pep. of last 100 aa of the Hu RFXANK (GenBank PID 4506499) conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-22855 detects RFXANK in Human samples.
PA1-22855 has been successfully used in Immunoprecipitation and Western Blot procedures.
PA1-22855 immunogen corresponds to Syn. Pep. of last 100 aa of the Hu RFXANK (GenBank PID 4506499) conjugated to KLH.
MHC class II deficiency is an autosomal recessive immunodeficiency syndrome resulting from defects in transacting factors essential for transcription of MHC class II genes. There are 4 genetic complementation groups (A, B, C, and D), reflecting the existence of 4 MHC class II regulators. Group B is characterized by a defect in RFX binding, and although it accounts for the majority of patients, the factor defective in group B remained unknown until Masternak et al. (1998) isolated RFX by a novel single-step DNA-affinity purification approach and identified RFXANK, the gene encoding the third subunit of RFX. The gene was symbolized RFXANK because the protein contains 3 ankyrin repeats.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ANKRA1; Ankyrin repeat family A protein 1; ankyrin repeat-containing regulatory factor X-associated protein; BLS; DNA-binding protein RFXANK; F14150_1; MGC138628; Regulatory factor X subunit B; Regulatory factor X-associated ankyrin-containing protein; RFX-B; RFX-Bdelta4
Gene Aliases: ANKRA1; BLS; F14150_1; RFX-B; RFXANK; RFXB
UniProt ID: (Human) O14593
Entrez Gene ID: (Human) 8625
Molecular Function: transcription factor
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