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Invitrogen
RGMB Polyclonal Antibody
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FIGURE: 1 / 1
RGMB Antibody (PA5-47516) in IHC (F)
Immunohistochemical analysis of RGMB in perfusion fixed frozen sections of mouse brain (trigeminal ganglia). Samples were incubated in RGMB polyclonal antibody (Product # PA5-47516) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Product Details
PA5-47516
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-B Gly49-Ser414
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) RGM-A and rmRGM-C is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Repulsive Guidance Molecule B (RGMB) is primarily expressed in the central nervous system. RGM A and B both appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGMA appears in the neuroepithelium, and only RGMB in the peripheral nervous system. During development, both RGMA and B appear also in lung and in limb cartilage, while RGMB has additional expression domains in pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRAGON; DRG11-responsive axonal guidance and outgrowth of neurite; Repulsive guidance molecule B; RGM domain family member B; RGM domain family, member B
Gene Aliases: 1110059F19Rik; DRAGON; Rgmb
UniProt ID: (Mouse) Q7TQ33
Entrez Gene ID: (Mouse) 68799
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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