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Invitrogen
RIP3 Polyclonal Antibody
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FIGURE: 1 / 8
RIP3 Antibody (PA1-41533) in ICC/IF
Immunocytochemistry analysis of RIP3 in HeLa cells. Samples were incubated with RIP3 polyclonal antibody (Product # PA1-41533) using a dilution of 1:200 dilution overnight at 4°C followed by Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. Alpha tubulin was used at a 1:1,000 dilution as a co-stain and detected with DyLight 550 (Red) at a 1:500 dilution. Nuclei were detected with DAPI (Blue) at 2.0 µg/mL in 1X PBS. Cells were... View More
Product Details
PA1-41533
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 497-518 of human RIPK3/RIP3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Sequence homology: Chimpanzee (100%). This antibody is not suitable for testing of mouse RIP3.
Immunogen sequence: QEGPKDPEAW SRPQ
Target Information
RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, which contains a C-terminal domain unique from other RIP family members. RIPK3 is predominantly localized to the cytoplasm, and specifically, are colocalized in the mitochondrion which can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. RIPK3 is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor and caspase-8 prevents RIPK3-dependent necrosis without inducing apoptosis by functioning in a proteolytically active complex with CFLAR and that this complex is required for the protective function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine-threonine kinase 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3
Gene Aliases: RIP3; RIPK3
UniProt ID: (Human) Q9Y572
Entrez Gene ID: (Human) 11035
regulation of T cell mediated cytotoxicity
regulation of adaptive immune response
cellular protein modification process
signal transduction
I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatase activity
activation of protein kinase activity
regulation of interferon-gamma production
T cell differentiation in thymus
NIK/NF-kappaB signaling
T cell homeostasis
regulation of activated T cell proliferation
protein autophosphorylation
lymph node development
spleen development
thymus development
positive regulation of NF-kappaB transcription factor activity
protein homooligomerization
protein heterooligomerization
positive regulation of ligase activity
positive regulation of oxidoreductase activity
positive regulation of necroptotic process
regulation of activation-induced cell death of T cells
necroptotic process
positive regulation of protein deacetylation
apoptotic signaling pathway
amyloid fibril formation
positive regulation of reactive oxygen species metabolic process
regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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