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Invitrogen
RNASE3 Polyclonal Antibody
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FIGURE: 1 / 1
RNASE3 Antibody (PA5-89860) in WB
Western blot analysis of RNASE3 in extracts of various cell lines. Samples were incubated with RNASE3 Polyclonal antibody (Product # PA5-89860) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-89860
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-160 of human RNASE3 (NP_0029262)
Conjugate
Form
Liquid
Concentration
1.01 mg/mL
Amount
101 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-549, HepG2
Immunogen sequence: RSRFRVPLLH CDLINPGAQN ISNCTYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I
Target Information
ECP (eosinophil cationic protein), also known as RNS3 or RNASE3 (ribonuclease, RNase A family, 3), is a 160 amino acid potent cytotoxic secretory protein that belongs to the pancreatic ribonuclease family. ECP localizes to the granule matrix of the eosinophil and is involved in the immune response system. ECP is a cytotoxin, neurotoxin and helminthotoxin that is secreted by activated human eosinophils. ECP exhibits anti-parasitic, antibacterial and ribonucleolytic activities. It has been suggested that ECP induces the neurotoxic effect known as the Gordon phenomenon, a syndrome manifested by ataxia, muscular rigidity, paralysis and tremor that may lead to death. ECP is considered a marker of eosinophilic inflammation and high levels have been found in cases of active asthma and other allergic diseases.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytotoxic ribonuclease; ECP; Eosinophil cationic protein; eosinophil cationic protein-like; Ribonuclease 3; ribonuclease, RNase A family, 3; RNase 3; unnamed protein product
Gene Aliases: ECP; RAF1; RNASE3; RNS3
UniProt ID: (Human) P12724
Entrez Gene ID: (Rat) 100361866, (Human) 6037
innate immune response in mucosa
RNA catabolic process
chemotaxis
antibacterial humoral response
defense response to bacterium
induction of bacterial agglutination
innate immune response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
antimicrobial humoral immune response mediated by antimicrobial peptide
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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