Proteintech

ROBO1 Polyclonal Antibody

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ROBO1 Antibody (20219-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 20219-1-AP) (ROBO1-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Product Details

20219-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to ROBO1.

Target Information

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway.

Advances in clinical and experimental medicine : official organ Wroclaw Medical University

Shang H,Xu A,Yan H,Xu D,Zhang J,Fang X

Sat Jul 01 00:00:00 UTC 2023

AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.

Molecular cell

Cho H,Abshire ET,Popp MW,Pröschel C,Schwartz JL,Yeo GW,Maquat LE

Thu Aug 04 00:00:00 UTC 2022

RNA m6A reader IGF2BP3 promotes metastasis of triple-negative breast cancer via SLIT2 repression.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jiang T,He X,Zhao Z,Zhang X,Wang T,Jia L

Tue Nov 01 00:00:00 UTC 2022

Role of Slit2 upregulation in recurrent miscarriage through regulation of stromal decidualization.

Placenta

Chen J,Liu J,Gao S,Qiu Y,Wang Y,Zhang Y,Gao L,Qi G,Wu Y,Lash GE,Li P

Fri Jan 01 00:00:00 UTC 2021

Mechanistic Targets of Diallyl Trisulfide in Human Breast Cancer Cells Identified by RNA-seq Analysis.

Journal of cancer prevention

Hahm ER,Kim SH,Mathan SV,Singh RP,Singh SV

Wed Jun 30 00:00:00 UTC 2021

MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma.

Aging

Zhang Z,Guo X,Guo X,Yu R,Qian M,Wang S,Gao X,Qiu W,Guo Q,Xu J,Chen Z,Wang H,Qi Y,Zhao R,Xue H,Li G

Mon Feb 01 00:00:00 UTC 2021

Activation of EPHA2-ROBO1 Heterodimer by SLIT2 Attenuates Non-canonical Signaling and Proliferation in Squamous Cell Carcinomas.

iScience

Srivastava S,Pang KM,Iida M,Nelson MS,Liu J,Nam A,Wang J,Mambetsariev I,Pillai R,Mohanty A,McDaniel N,Behal A,Kulkarni P,Wheeler DL,Salgia R

Fri Nov 20 00:00:00 UTC 2020

A crucial role for Arf6 in the response of commissural axons to Slit.

Development (Cambridge, England)

Kinoshita-Kawada M,Hasegawa H,Hongu T,Yanagi S,Kanaho Y,Masai I,Mishima T,Chen X,Tsuboi Y,Rao Y,Yuasa-Kawada J,Wu JY

Mon Feb 04 00:00:00 UTC 2019

A crucial role for Arf6 in the response of commissural axons to Slit.

Development (Cambridge, England)

Kinoshita-Kawada M,Hasegawa H,Hongu T,Yanagi S,Kanaho Y,Masai I,Mishima T,Chen X,Tsuboi Y,Rao Y,Yuasa-Kawada J,Wu JY

Mon Feb 04 00:00:00 UTC 2019

Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.

Nature cell biology

Simonetti B,Paul B,Chaudhari K,Weeratunga S,Steinberg F,Gorla M,Heesom KJ,Bashaw GJ,Collins BM,Cullen PJ

Tue Oct 01 00:00:00 UTC 2019

Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.

Angiogenesis

Liu J,Hou W,Guan T,Tang L,Zhu X,Li Y,Hou S,Zhang J,Chen H,Huang Y

Tue May 01 00:00:00 UTC 2018

Slit2/Robo1 signaling is involved in angiogenesis of glomerular endothelial cells exposed to a diabetic-like environment.

Angiogenesis

Liu J,Hou W,Guan T,Tang L,Zhu X,Li Y,Hou S,Zhang J,Chen H,Huang Y

Tue May 01 00:00:00 UTC 2018

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Histopathology

Li P,Shi Y,Shuai H,Cai Y,Lu W,Wang G,Gao L,Wang L,Fan X,Yang X

Sun Oct 01 00:00:00 UTC 2017

Alterted SLIT2/ROBO1 signalling is linked to impaired placentation of missed and threatened miscarriage in early pregnancy.

Histopathology

Li P,Shi Y,Shuai H,Cai Y,Lu W,Wang G,Gao L,Wang L,Fan X,Yang X

Sun Oct 01 00:00:00 UTC 2017

MicroRNA-219-5p exerts tumor suppressor function by targeting ROBO1 in glioblastoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Jiang Y,Yin L,Jing H,Zhang H

Sun Nov 01 00:00:00 UTC 2015

USP33, a new player in lung cancer, mediates Slit-Robo signaling.

Protein & cell

Wen P,Kong R,Liu J,Zhu L,Chen X,Li X,Nie Y,Wu K,Wu JY

Mon Sep 01 00:00:00 UTC 2014

USP33, a new player in lung cancer, mediates Slit-Robo signaling.

Protein & cell

Wen P,Kong R,Liu J,Zhu L,Chen X,Li X,Nie Y,Wu K,Wu JY

Mon Sep 01 00:00:00 UTC 2014

USP33, a new player in lung cancer, mediates Slit-Robo signaling.

Protein & cell

Wen P,Kong R,Liu J,Zhu L,Chen X,Li X,Nie Y,Wu K,Wu JY

Mon Sep 01 00:00:00 UTC 2014

USP33, a new player in lung cancer, mediates Slit-Robo signaling.

Protein & cell

Wen P,Kong R,Liu J,Zhu L,Chen X,Li X,Nie Y,Wu K,Wu JY

Mon Sep 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1

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Gene Aliases: AW494633; AW742721; DUTT1; Gm310; ROBO1; SAX3

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UniProt ID: (Human) Q9Y6N7, (Rat) O55005, (Mouse) O89026

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Entrez Gene ID: (Human) 6091, (Rat) 58946, (Mouse) 19876

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Function(s)

protein binding axon guidance receptor activity LRR domain binding identical protein binding scaffold/adaptor protein

Process(es)

cell migration involved in sprouting angiogenesis activation of cysteine-type endopeptidase activity involved in apoptotic process cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules nervous system development axon guidance heart development axon midline choice point recognition chemorepulsion involved in postnatal olfactory bulb interneuron migration negative regulation of cell migration negative regulation of mammary gland epithelial cell proliferation positive regulation of Rho protein signal transduction Roundabout signaling pathway positive regulation of axonogenesis negative regulation of negative chemotaxis negative regulation of chemokine-mediated signaling pathway central nervous system development negative regulation of cell proliferation spinal cord development olfactory bulb interneuron development regulation of dendrite morphogenesis mammary duct terminal end bud growth chemotaxis multicellular organism development cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ROBO1 Polyclonal Antibody

ROBO1 Antibody (20219-1-AP) in WB

Product Details

20219-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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