Search
Invitrogen
ROBO1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
PA5-65752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human ROBO1. Recombinant protein control fragment (Product #RP-106430).
Conjugate
Form
Liquid
Concentration
0.10 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: REVLDGRQVVD MRTNPGDPRE AQEQQNDGKG RGNKAAKRDL PPAKTHLIQE DILPYCRPTF PTSNNPRDPS SSSSMSSRGS GSRQREQANV GRRNIAEMQV LG
Highest antigen sequence identity to the following orthologs - mouse 92%, rat 89%.
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; H-Robo1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; unnamed protein product
Gene Aliases: CPHD8; DUTT1; NORS; NYS8; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7
Entrez Gene ID: (Human) 6091
cell migration involved in sprouting angiogenesis
heart induction
outflow tract septum morphogenesis
aortic valve morphogenesis
pulmonary valve morphogenesis
endocardial cushion formation
chemotaxis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
nervous system development
axon guidance
brain development
positive regulation of gene expression
negative regulation of gene expression
axon midline choice point recognition
chemorepulsion involved in postnatal olfactory bulb interneuron migration
cell differentiation
negative regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of mammary gland epithelial cell proliferation
positive regulation of Rho protein signal transduction
tube development
Roundabout signaling pathway
aorta development
positive regulation of MAPK cascade
positive regulation of Notch signaling pathway
positive regulation of axonogenesis
negative regulation of negative chemotaxis
ventricular septum morphogenesis
negative regulation of chemokine-mediated signaling pathway
positive regulation of vascular endothelial growth factor signaling pathway
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support