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Invitrogen
ROBO1 Recombinant Mouse Monoclonal Antibody (10E2-R)
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FIGURE: 1 / 3
ROBO1 Antibody (MA5-44934) in IHC (P)
Immunohistochemistry analysis of ROBO1 in paraffin-embedded human placenta tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with ROBO1 Monoclonal antibody (Product # MA5-44934) using a dilution of 1:200 for 1 hour at room te... View More
Product Details
MA5-44934
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10E2-R
Immunogen
Recombinant protein within Human ROBO1 aa 51-300/1,651.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Deleted in U twenty twenty; FLJ21882; H-Robo-1; H-Robo1; HGNC:10249; MGC131599; MGC133277; roundabout 1; Roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; unnamed protein product
Gene Aliases: AW494633; AW742721; CPHD8; DUTT1; Gm310; NORS; NYS8; ROBO1; SAX3
UniProt ID: (Human) Q9Y6N7, (Rat) O55005, (Mouse) O89026
Entrez Gene ID: (Human) 6091, (Rat) 58946, (Mouse) 19876
cell migration involved in sprouting angiogenesis
heart induction
outflow tract septum morphogenesis
aortic valve morphogenesis
pulmonary valve morphogenesis
endocardial cushion formation
chemotaxis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
nervous system development
axon guidance
brain development
positive regulation of gene expression
negative regulation of gene expression
axon midline choice point recognition
chemorepulsion involved in postnatal olfactory bulb interneuron migration
cell differentiation
negative regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of mammary gland epithelial cell proliferation
positive regulation of Rho protein signal transduction
tube development
Roundabout signaling pathway
aorta development
positive regulation of MAPK cascade
positive regulation of Notch signaling pathway
positive regulation of axonogenesis
negative regulation of negative chemotaxis
ventricular septum morphogenesis
negative regulation of chemokine-mediated signaling pathway
positive regulation of vascular endothelial growth factor signaling pathway
activation of cysteine-type endopeptidase activity involved in apoptotic process
central nervous system development
negative regulation of cell proliferation
spinal cord development
olfactory bulb interneuron development
regulation of dendrite morphogenesis
mammary duct terminal end bud growth
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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