Invitrogen

ROCK2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (6)
Advanced Verification (1)

FIGURE: 1 / 7

ROCK2 Antibody (PA5-78291) in WB

Western blot was performed using Anti-ROCK2 Polyclonal Antibody (Product # PA5-78291) and a 180 kDa corresponding to ROCK2 along with an uncharacterized band around 90 kDa was observed across cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), MDA-MB-231 (Lane 4), U-87 MG (Lane 5) and C1C12 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLo... View More

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ROCK2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ROCK2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ROCK2 Rabbit Polyclonal Antibody (Product # PA5-78291) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ROCK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — ROCK2 Polyclonal Antibody detects ROCK2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ROCK2 Polyclonal Antibody (Product # PA5-78291) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

ROCK2 Antibody — Antibody specificity was demonstrated by shRNA mediated knockdown of target protein. 293T cells were transfected with ROCK2 shRNA and decrease in signal intensity was observed in Western blotting using Anti-ROCK2 Polyclonal Antibody (Product # PA5-78291). {KD}

Product Details

PA5-78291

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ROCK2. The exact sequence is proprietary.

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Conjugate

Unconjugated

Form

Liquid

Concentration

0.192 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2736535

Product Specific Information

Positive Control: 293T, A431, HeLa, HepG2

Predicted Reactivity: Rat (100%), Rhesus Monkey (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; ROCK II; ROCK-II; rockii

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Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROKalpha

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UniProt ID: (Human) O75116, (Mouse) P70336

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Entrez Gene ID: (Human) 9475, (Mouse) 19878

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Molecular Function: non-receptor serine/threonine protein kinase

Function(s)

cytokinesis positive regulation of protein phosphorylation protein phosphorylation smooth muscle contraction I-kappaB kinase/NF-kappaB signaling Rho protein signal transduction positive regulation of endothelial cell migration positive regulation of gene expression positive regulation of centrosome duplication negative regulation of angiogenesis regulation of cell adhesion cortical actin cytoskeleton organization regulation of actin cytoskeleton organization negative regulation of myosin-light-chain-phosphatase activity regulation of circadian rhythm regulation of keratinocyte differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway rhythmic process centrosome duplication regulation of stress fiber assembly regulation of focal adhesion assembly cellular response to testosterone stimulus establishment of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly regulation of establishment of cell polarity regulation of cell motility neural tube closure extrinsic apoptotic signaling pathway via death domain receptors phosphorylation actin cytoskeleton organization intracellular signal transduction dendrite morphogenesis

Process(es)

protein serine/threonine kinase activity structural molecule activity protein binding ATP binding Rho GTPase binding poly(A) RNA binding metal ion binding Rho-dependent protein serine/threonine kinase activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ROCK2 Polyclonal Antibody

ROCK2 Antibody (PA5-78291) in WB

Product Details

PA5-78291

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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