Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is colon surface epithelium.
Rabbit anti Human CD136 antibody detects CD136, a receptor tyrosine kinase also known as Macrophage stimulating 1 receptor (MST1R) and RON (Recepteur d'origine nantais).
Macrophage-stimulatin 1 receptor (MST1R/Ron), a HGF Receptor/MET-type protein kinase, mediates the biological activities of macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival. The protein is a membrane-spanning, disulfide-linked heterodimer, which results from cleavage of a glycosylated precursor into 35-kD (alpha) and 150-kD (beta) subunits. Ligand binding results in tyrosine phosphorylation of the beta chain. In knockout studies, MST1R/RON (-/-) mice failed to survive past the periimplantation period. The MST1R/RON gene has been mapped to 3p21, a region of frequent deletion or mutation in small cell lung and renal carcinoma, and has been implicated in the progression of several epithelial cancers. MST1R/Ron expression has been documented in many normal human tissues. ESTs have been isolated from several tissue libraries, including normal colon, mouth, prostate, and testis and cancerous colon, prostate, stomach, and uteru.
Protein Aliases: c-met-related tyrosine kinase; CD136; CDw136; Macrophage-stimulating protein receptor; Macrophage-stimulating protein receptor alpha chain; Macrophage-stimulating protein receptor beta chain; MSP receptor; MST1R variant RON30; MST1R variant RON62; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; RON variant E2E3
Gene Aliases: CD136; CDw136; MST1R; PTK8; RON
UniProt ID: (Human) Q04912
Entrez Gene ID: (Human) 4486
Molecular Function: transmembrane signal receptor