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Agrisera
RPB1/RNA polymerase II subunit B1 Polyclonal Antibody
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FIGURE: 1 / 1
RPB1/RNA polymerase II subunit B1 Antibody (AS11-1804) in ChIP
Chromatin immunoprecipitation (ChIP). Chromatin immunoprecipitation was performed as described (Bowler et al. (2004), Plant Journal) with modifications. Sonic and IP buffer were prepared as described (Kaufmann et al. (2010)), Nature Protocols). Chromatin pellet was sonicated at 4 °C with a Diagenode Bio Ruptor set at high intensity for 10 min (30 sec on, 30 sec off intervals) to obtain 250-500 bp DNA fragme... View More
Product Details
AS11-1804
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
RPB1 encodes the largest catalytic subunit of eukaryotic RNA polymerase II (the POLR2A gene product), a predominantly nuclear, chromatin-associated enzyme complex responsible for DNA-templated transcription of protein-coding genes into precursor mRNA. The RPB1 polypeptide forms the core of the polymerase active-center framework and, together with other polymerase subunits, contributes to the DNA-binding cleft that positions the template strand for RNA synthesis. A defining structural feature is its carboxy-terminal domain (CTD), a low-complexity tail composed of tandem heptapeptide repeats with the consensus sequence YSPTSPS; in mammals the CTD comprises 52 repeats and is essential for polymerase activity. Dynamic post-translational modification of CTD residues-especially phosphorylation of Ser2 and Ser5 across the repeats-coordinates transitions through initiation, elongation, and termination and functionally couples transcription to co-transcriptional RNA processing by recruiting and organizing factors required for 5-prime capping, splicing, and 3-prime end cleavage/polyadenylation on nascent transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: At4g35800; F4B14.70; NRPB1; RNA_POL_II_LS; RNA_POL_II_LSRNA_POL_II_LS; RPB1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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