Proteintech

RPLP0 Polyclonal Antibody

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RPLP0 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11290-2-AP (RPLP0 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RPLP0 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 11290-2-AP (RPLP0 antibody) at dilution of 1:100 (under 10x lens).

RPLP0 Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 11290-2-AP (RPLP0 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RPLP0 Antibody in Immunoprecipitation (IP) — IP result of anti-RPLP0 (IP:11290-2-AP, 4 ug; Detection:11290-2-AP 1:500) with HeLa cells lysate 3200 ug.

Product Details

11290-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

ELISA (ELISA)

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPLP0 Fusion Protein Ag1829 (1-317 aa encoded by BC009867)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MPREDRATW KSNYFLKIIQ LLDDYPKCFI VGADNVGSKQ MQQIRMSLRG KAVVLMGKNT MMRKAIRGHL ENNPALEKLL PHIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ LIKTGDKVGA SEATLLNMLN ISPFSFGLVI QQVFDNGSIY NPEVLDITEE TLHSRFLEGV RNVASVCLQI GYPTVASVPH SIINGYKRVL ALSVETDYTF PLAEKVKAFL ADPSAFVAAA PVAAATTAAP AAAAAPAKVE AKEESEESDE DMGFGLFD (1-317 aa encoded by BC009867)

Target Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rpl3l gene deletion in mice reduces heart weight over time.

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Grimes KM,Prasad V,Huo J,Kuwabara Y,Vanhoutte D,Baldwin TA,Bowers SLK,Johansen AKZ,Sargent MA,Lin SJ,Molkentin JD

Sat Feb 04 00:00:00 EST 2023

Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells.

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Datta C,Truesdell SS,Wu KQ,Bukhari SIA,Ngue H,Buchanan B,Le Tonqueze O,Lee S,Kollu S,Granovetter MA,Boukhali M,Kreuzer J,Batool MS,Balaj L,Haas W,Vasudevan S

Fri Oct 28 00:00:00 EDT 2022

Mitochondrial ROS-mediated ribosome stalling and GCN2 activation are partially involved in 1-nitropyrene-induced steroidogenic inhibition in testes.

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Thu Sep 01 00:00:00 EDT 2022

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Tue Aug 16 00:00:00 EDT 2022

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

Tue Feb 16 00:00:00 EST 2021

System biology analysis reveals the role of voltage-dependent anion channel in mitochondrial dysfunction during non-alcoholic fatty liver disease progression into hepatocellular carcinoma.

Cancer science

Zhu Y,Zhang C,Xu F,Zhao M,Bergquist J,Yang C,Liu X,Tan Y,Wang X,Li S,Jiang W,Ong Q,Lu L,Mi J,Tian G

Sun Nov 01 00:00:00 EDT 2020

Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1α,25-dihydroxyvitamin D₃.

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Mon Jul 01 00:00:00 EDT 2019

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Thu Sep 07 00:00:00 EDT 2017

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Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.

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Tue Oct 04 00:00:00 EDT 2016

Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells.

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Sun Nov 01 00:00:00 EDT 2015

Differential Contributions of Alcohol and the Nicotine-Derived Nitrosamine Ketone (NNK) to Insulin and Insulin-Like Growth Factor Resistance in the Adolescent Rat Brain.

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Sun Nov 01 00:00:00 EDT 2015

Potential Role of Phosphorylation as a Regulator of Aspartyl-(asparaginyl)-β-hydroxylase: Relevance to Infiltrative Spread of Human Hepatocellular Carcinoma.

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Borgas DL,Gao JS,Tong M,de la Monte SM

Tue Sep 01 00:00:00 EDT 2015

Potential Role of Phosphorylation as a Regulator of Aspartyl-(asparaginyl)-β-hydroxylase: Relevance to Infiltrative Spread of Human Hepatocellular Carcinoma.

Liver cancer

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Tue Sep 01 00:00:00 EDT 2015

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Mon Dec 01 00:00:00 EST 2014

ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction.

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Wed Aug 01 00:00:00 EDT 2012

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Genomic convergence among ERRα, PROX1, and BMAL1 in the control of metabolic clock outputs.

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Dufour CR,Levasseur MP,Pham NH,Eichner LJ,Wilson BJ,Charest-Marcotte A,Duguay D,Poirier-Héon JF,Cermakian N,Giguère V

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miR-378(∗) mediates metabolic shift in breast cancer cells via the PGC-1β/ERRγ transcriptional pathway.

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The homeobox protein Prox1 is a negative modulator of ERR{alpha}/PGC-1{alpha} bioenergetic functions.

Genes & development

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The Journal of cell biology

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Mon Nov 17 00:00:00 EST 2008

Nucleolar protein B23/nucleophosmin regulates the vertebrate SUMO pathway through SENP3 and SENP5 proteases.

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Bioinformatics

Protein Aliases: 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0; Large ribosomal subunit protein uL10; MGC107165; MGC107166; MGC111226; MGC88175; neutral ribosomal phosphoprotein P0; OTTMUSP00000015585; ribosomal protein, large, P0; rplRP

Gene Aliases: 36B4; Arbp; L10E; LP0; P0; PRLP0; RPLP0; RPP0

UniProt ID: (Human) P05388, (Mouse) P14869, (Rat) P19945

Entrez Gene ID: (Human) 6175, (Mouse) 11837, (Rat) 64205

Molecular Function: ribosomal protein translational protein