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Invitrogen
RPSA Monoclonal Antibody (MLuC5)
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FIGURE: 1 / 5
RPSA Antibody (MA5-12840) in ICC/IF
Immunofluorescent analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Laminin Receptor monoclonal antibody (Product # MA5-12840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated... View More
Product Details
MA5-12840
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MLuC5
Immunogen
Small cell lung carcinoma N592 live cells
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-12840 targets Laminin Receptor in FACS, WB, ICC/IF, and IHC (P) applications and shows reactivity with Human samples.
The MA5-12840 immunogen is small cell lung carcinoma N592 live cells.
Target Information
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 37 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin-binding protein; ICAS; LAMBR; laminin receptor; Laminin receptor 1; laminin receptor 1 (67kD, ribosomal protein SA); laminin-binding protein; Laminin-binding protein precursor p40; LamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; rssa; SA; Small ribosomal subunit protein uS2; unnamed protein product; uS2
Gene Aliases: 37LRP; 67LR; ICAS; LAMBR; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; NEM/1CHD4; p40; RPSA; SA; uS2
UniProt ID: (Human) P08865
Entrez Gene ID: (Human) 3921
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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