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Invitrogen

RUNX1/RUNX1T1 Polyclonal Antibody

1 Published Figure
2 References

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Product Details

PA5-40076

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

ELISA (ELISA)

1:500
-

Immunoprecipitation (IP)

-
View 1 publication 1 publication

ChIP assay (ChIP)

4 µL
View 1 publication 1 publication

ChIP-sequencing (ChIP-Seq)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH-conjugated synthetic peptide corresponding to human AML1-ETO fusion protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608182

Target Information

AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in all tissues examined except brain and heart, and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: acute myelogenous leukemia 1 translocation 1, cyclin-D related; Acute myeloid leukemia 1 protein; aml1; aml1 oncogene; AML1-ETO fusion; AML1-ETO fusion protein; AML1-EVI-1 fusion protein; CBF-alpha-2; CBFA2T1; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related; Cyclin-D-related protein; Eight twenty one protein; ETO; fusion gene; mutant RUNX1; myeloid translocation gene on 8q22; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; Protein CBFA2T1; Protein ETO; Protein MTG8; putative transcription factor; runt related transcription factor 1; runt related transcription factor 1; translocated to, 1 (cyclin D related); Runt-related transcription factor 1; runt-related transcription factor 1; translocated to, 1 (cyclin D-related); RUNX1 translocation partner 1; RUNX1 with skipped exon 5; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; unnamed protein product; Zinc finger MYND domain-containing protein 2

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Gene Aliases: AML1; AML1-EVI-1; AML1-MTG8; AML1T1; AMLCR1; CBF2alpha; CBFA2; CBFA2T1; CDR; ETO; EVI-1; MTG8; PEBP2aB; PEBP2alpha; RUNX1; RUNX1T1; ZMYND2

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UniProt ID: (Human) Q01196, (Human) Q06455

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Entrez Gene ID: (Human) 861, (Human) 862

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding protein binding ATP binding protein homodimerization activity protein heterodimerization activity DNA-binding transcription factor binding sequence-specific double-stranded DNA binding transcription corepressor activity zinc ion binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification chondrocyte differentiation myeloid leukocyte differentiation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter regulation of plasminogen activation hemopoiesis myeloid cell differentiation neuron differentiation negative regulation of granulocyte differentiation positive regulation of granulocyte differentiation positive regulation of interleukin-2 production positive regulation of collagen biosynthetic process negative regulation of CD4-positive, alpha-beta T cell differentiation positive regulation of CD8-positive, alpha-beta T cell differentiation regulation of cell differentiation positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter peripheral nervous system neuron development positive regulation of developmental process positive regulation of multicellular organismal process regulation of cardiac muscle cell proliferation cardiac muscle tissue regeneration hematopoietic stem cell proliferation positive regulation of extracellular matrix organization regulation of connective tissue replacement regulation of multicellular organismal development transcription, DNA-templated negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated
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