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Invitrogen
RUNX1/RUNX1T1 Polyclonal Antibody
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FIGURE: 1 / 5
RUNX1/RUNX1T1 Antibody (PA5-40076) in ChIP-seq
ChIP assays were performed on Kasumi-1 cells using a AML1-ETO polyclonal antibody (Product # PA5-40076) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 µl of antibody were used per ChIP experiment. QPCR was performed on primers specific for the FUT7, NFE2 and OGG1 genes. The IP'd DNA of 6 ChIPs was pooled and analyzed with a Genome Analyzer. The 32 bp tags were aligned t... View More
Product Details
PA5-40076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide corresponding to human AML1-ETO fusion protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in all tissues examined except brain and heart, and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acute myelogenous leukemia 1 translocation 1, cyclin-D related; Acute myeloid leukemia 1 protein; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha-2; CBFA2T1 isoform r1t1-11a61; CBFA2T1 isoform r1t1-11a62; CBFA2T1 isoform r1t1-11a63; CBFA2T1 isoform r1t1-11a64; CBFA2T1 isoform r1t1-11a65; CBFA2T1 isoform r1t1-7a47; CBFA2T1 isoform r1t1-7a48; CBFA2T1 isoform r1t1-7a49; CBFA2T1 isoform r1t1-7a50; CBFA2T1 isoform r1t1-7a51; CBFA2T1 isoform r1t1-7a52; CBFA2T1 isoform r1t1-7d53; CBFA2T1 isoform r1t1-7d54; CBFA2T1 isoform r1t1-7d55; CBFA2T1 isoform r1t1-7d56; CBFA2T1 isoform r1t1-8a57; CBFA2T1 isoform r1t1-8a58; CBFA2T1 isoform r1t1-8a59; CBFA2T1 isoform r1t1-8a60; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related; Cyclin-D-related protein; Eight twenty one protein; myeloid translocation gene on 8q22; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; Protein CBFA2T1; Protein ETO; Protein MTG8; runt related transcription factor 1; translocated to, 1 (cyclin D related); Runt-related transcription factor 1; runt-related transcription factor 1; translocated to, 1 (cyclin D-related); RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor; Zinc finger MYND domain-containing protein 2
Gene Aliases: AML1; AML1-EVI-1; AML1-MTG8; AML1T1; AMLCR1; CBF2alpha; CBFA2; CBFA2T1; CDR; ETO; EVI-1; MTG8; PEBP2aB; PEBP2alpha; RUNX1; RUNX1T1; ZMYND2
UniProt ID: (Human) Q01196, (Human) Q06455
Entrez Gene ID: (Human) 861, (Human) 862
regulatory region DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
ATP binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
metal ion binding
transcription cofactor
DNA-binding transcription factor
immunoglobulin fold transcription factor
Runt transcription factor
gene-specific transcriptional regulator
ossification
chondrocyte differentiation
transcription from RNA polymerase II promoter
hemopoiesis
positive regulation of granulocyte differentiation
positive regulation of interleukin-2 production
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
hematopoietic stem cell proliferation
generation of precursor metabolites and energy
transcription, DNA-templated
negative regulation of fat cell differentiation
negative regulation of transcription, DNA-templated
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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