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RUNX1 (middle) Polyclonal Antibody

RUNX1 (middle) Antibody in Immunocytochemistry (ICC/IF)

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RUNX1 (middle) Antibody (25315-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 25315-1-AP (RUNX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RUNX1 (middle) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RUNX1 (middle) Antibody in Western Blot (WB)

RUNX1 (middle) Antibody in Immunoprecipitation (IP)

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Product Details

25315-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RUNX1 (middle) Fusion Protein Ag17838 (214-277 aa encoded by BC136381)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TKPGSLSFS ERLSELEQLR RTAMRVSPHH PAPTPNPRAS LNHSTAFNPQ PQSQMQDTRQ IQPSP (214-277 aa encoded by BC136381)

Target Information

RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting UBE2T Potentiates Gemcitabine Efficacy in Pancreatic Cancer by Regulating Pyrimidine Metabolism and Replication Stress.

Gastroenterology

Jiang X,Ma Y,Wang T,Zhou H,Wang K,Shi W,Qin L,Guan J,Li L,Long B,Wang J,Guan X,Ye H,Yang J,Yu Z,Jiao Z

Thu Jun 01 00:00:00 EDT 2023

miR-9 targeting RUNX1 improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS.

International immunopharmacology

Xiao C,Li Q,Xiao J,Chen X,Yuan J,Li S,Li W,Gao D,Li L,Liu Y,Shen F

Sat Jul 01 00:00:00 EDT 2023

Targeting TLR2/Rac1/cdc42/JNK Pathway to Reveal That Ruxolitinib Promotes Thrombocytopoiesis.

International journal of molecular sciences

Yang S,Tang X,Wang L,Ni C,Wu Y,Zhou L,Zeng Y,Zhao C,Wu A,Wang Q,Xu X,Wang Y,Chen R,Zhang X,Zou L,Huang X,Wu J

Sat Dec 17 00:00:00 EST 2022

Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.

Journal of cellular physiology

Dubey S,Dubey PK,Umeshappa CS,Ghebre YT,Krishnamurthy P

Fri Apr 01 00:00:00 EDT 2022

Pharmacologic targeting of the P-TEFb complex as a therapeutic strategy for chronic myeloid leukemia.

Cell communication and signaling : CCS

Qing Y,Wang X,Wang H,Hu P,Li H,Yu X,Zhu M,Wang Z,Zhu Y,Xu J,Guo Q,Hui H

Mon Aug 09 00:00:00 EDT 2021

Angiotensin II-Induced Long Non-Coding RNA Alivec Regulates Chondrogenesis in Vascular Smooth Muscle Cells.

Cells

Samara VA,Das S,Reddy MA,Tanwar VS,Stapleton K,Leung A,Abdollahi M,Ganguly R,Lanting L,Natarajan R

Sat Oct 09 00:00:00 EDT 2021

ASCL1 represses a SOX9⁺ neural crest stem-like state in small cell lung cancer.

Genes & development

Olsen RR,Ireland AS,Kastner DW,Groves SM,Spainhower KB,Pozo K,Kelenis DP,Whitney CP,Guthrie MR,Wait SJ,Soltero D,Witt BL,Quaranta V,Johnson JE,Oliver TG

Tue Jun 01 00:00:00 EDT 2021

ASCL1 represses a SOX9⁺ neural crest stem-like state in small cell lung cancer.

Genes & development

Olsen RR,Ireland AS,Kastner DW,Groves SM,Spainhower KB,Pozo K,Kelenis DP,Whitney CP,Guthrie MR,Wait SJ,Soltero D,Witt BL,Quaranta V,Johnson JE,Oliver TG

Tue Jun 01 00:00:00 EDT 2021

CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a.

Molecular cancer

Gan X,Zhu H,Jiang X,Obiegbusi SC,Yong M,Long X,Hu J

Fri Feb 28 00:00:00 EST 2020

CircMUC16 promotes autophagy of epithelial ovarian cancer via interaction with ATG13 and miR-199a.

Molecular cancer

Gan X,Zhu H,Jiang X,Obiegbusi SC,Yong M,Long X,Hu J

Fri Feb 28 00:00:00 EST 2020

Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics.

Cancer cell international

Zhang L,Wang W,Zhang S,Wang Y,Guo W,Liu Y,Wang Y,Zhang Y

Thu Apr 14 00:00:00 EDT 2022

RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.

Journal of hematology & oncology

Bera R,Chiu MC,Huang YJ,Lin TH,Kuo MC,Shih LY

Tue Oct 22 00:00:00 EDT 2019

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Immunity

Wang D,Diao H,Getzler AJ,Rogal W,Frederick MA,Milner J,Yu B,Crotty S,Goldrath AW,Pipkin ME

Tue Apr 17 00:00:00 EDT 2018

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Immunity

Wang D,Diao H,Getzler AJ,Rogal W,Frederick MA,Milner J,Yu B,Crotty S,Goldrath AW,Pipkin ME

Tue Apr 17 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; core binding factor alpha 2; core-binding factor runt domain alpha subunit 2 (acute myeloid leukemia 1 oncogene); Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; runt related transcription factor 1; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor

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Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; CBF2alpha; CBFA2; EVI-1; Pebp2a2; PEBP2aB; PEBP2alpha; Pebpa2b; RUNX1

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UniProt ID: (Human) Q01196, (Rat) Q63046

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Entrez Gene ID: (Human) 861, (Rat) 50662, (Mouse) 12394

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Function(s)

regulatory region DNA binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protein binding ATP binding molecular_function DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding protein homodimerization activity protein heterodimerization activity repressing transcription factor binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA-binding transcription factor immunoglobulin fold transcription factor Runt transcription factor gene-specific transcriptional regulator

Process(es)

ossification chondrocyte differentiation transcription from RNA polymerase II promoter hemopoiesis positive regulation of granulocyte differentiation positive regulation of interleukin-2 production positive regulation of angiogenesis positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter peripheral nervous system neuron development hematopoietic stem cell proliferation skeletal system development in utero embryonic development liver development myeloid progenitor cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated central nervous system development negative regulation of cell proliferation regulation of signal transduction myeloid cell differentiation neuron differentiation negative regulation of granulocyte differentiation hair follicle morphogenesis response to retinoic acid embryonic hemopoiesis behavioral response to pain neuron development definitive hemopoiesis regulation of hair follicle cell proliferation cellular response to transforming growth factor beta stimulus positive regulation of cell maturation positive regulation of progesterone secretion regulation of T cell anergy regulation of transcription from RNA polymerase II promoter positive regulation of interferon-gamma production negative regulation of transcription, DNA-templated

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RUNX1 (middle) Polyclonal Antibody

RUNX1 (middle) Antibody (25315-1-AP) in ICC/IF

Product Details

25315-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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