Immunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, Cy5 was performed using A-431 cells stained with EGFR (EP38Y) Rabbit Monoclonal Primary Antibody (MA514485). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2ug/ml rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody, Cy5 (A10523) was used at a concentration of 4ug/ml in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of EGFR (EP38Y) in the membrane (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||4 µg/ml|
|Immunofluorescence (IF)||4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 4 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 3 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
MUC13 interaction with receptor tyrosine kinase HER2 drives pancreatic ductal adenocarcinoma progression.
A10523 was used in immunocytochemistry to investigate the interaction between MUC13 and HER2
|Khan S,Sikander M,Ebeling MC,Ganju A,Kumari S,Yallapu MM,Hafeez BB,Ise T,Nagata S,Zafar N,Behrman SW,Wan JY,Ghimire HM,Sahay P,Pradhan P,Chauhan SC,Jaggi M||Oncogene (36:491)||2017|
Eye-independent, light-activated chromatophore expansion (LACE) and expression of phototransduction genes in the skin of Octopus bimaculoides.
A10523 was used in immunocytochemistry to elucidate how octopus skin senses light
|Ramirez MD,Oakley TH||The Journal of experimental biology (218:1513)||2015|
|Not Applicable||Not Cited||
Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism.
A10523 was used in immunocytochemistry and immunohistochemistry - paraffin section to elucidate how optineurin contributes to disease
|Shen WC,Li HY,Chen GC,Chern Y,Tu PH||Autophagy (11:685)||2015|
Identification of NOX2 regions for normal biosynthesis of cytochrome b558 in phagocytes highlighting essential residues for p22phox binding.
A10523 was used in immunocytochemistry to examine the effects of rare X-minus chronic granulomatous disease mutations
|Beaumel S,Grunwald D,Fieschi F,Stasia MJ||The Biochemical journal (464:425)||2014|
Claudin-6 enhances cell invasiveness through claudin-1 in AGS human adenocarcinoma gastric cancer cells.
A10523 was used in flow cytometry to ascertain how claudin-6 is associated with MMP-2 activation and cell invasiveness
|Torres-Martínez AC,Gallardo-Vera JF,Lara-Holguin AN,Montaño LF,Rendón-Huerta EP||Experimental cell research (350:226)||2017|
Unspecific binding of cRNA probe to plaques in two mouse models for Alzheimer's disease.
A10523 was used in immunohistochemistry - frozen section to study cadherin expression in brains affected by Alzheimer's disease
|Schaarschuch A,Redies C,Hertel N||Journal of negative results in biomedicine (15:null)||2016|
Lineage tracing of cells involved in atherosclerosis.
A10523 was used in immunohistochemistry - frozen section to identify the origin of vascular smooth muscle cells and macrophages within atherosclerosis lesions
|Albarrán-Juárez J,Kaur H,Grimm M,Offermanns S,Wettschureck N||Atherosclerosis (251:445)||2016|
Smooth muscle cell transplantation improves bladder contractile function in streptozocin-induced diabetic rats.
A10523 was used in immunohistochemistry - frozen section to discuss factors that contribute to defects observed in diabetic bladders
|Gopinath C,Ponsaerts P,Fransen E,Boeykens N,Pauwels P,Wyndaele JJ||Cytotherapy (15:869)||2013|
Progressive Muscle Cell Delivery as a Solution for Volumetric Muscle Defect Repair.
A10523 was used in immunohistochemistry to discuss factors to optimize the repair volumetric tissue defects
|Kim JH,Ko IK,Atala A,Yoo JJ||Scientific reports (6:null)||2016|
Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors.
A10523 was used in immunohistochemistry to assess the impact of heparan sulfate binding on retinal transduction by intravitreal-delivered adeno-associated viruses
|Boye SL,Bennett A,Scalabrino ML,McCullough KT,Van Vliet K,Choudhury S,Ruan Q,Peterson J,Agbandje-McKenna M,Boye SE||Journal of virology (90:4215)||2016|
Four Individually Identified Paired Dopamine Neurons Signal Reward in Larval Drosophila.
A10523 was used in immunohistochemistry to determine the function of different dopaminergic neurons using larval Drosophila
|Rohwedder A,Wenz NL,Stehle B,Huser A,Yamagata N,Zlatic M,Truman JW,Tanimoto H,Saumweber T,Gerber B,Thum AS||Current biology : CB (26:661)||2016|
Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae.
A10523 was used in immunohistochemistry to test if Drosophila neuropeptide F neurons are involved in classical olfactory conditioning
|Rohwedder A,Selcho M,Chassot B,Thum AS||The Journal of comparative neurology (523:2637)||2015|
|Not Applicable||Not Cited||
Saccade modulation by optical and electrical stimulation in the macaque frontal eye field.
A10523 was used in immunohistochemistry to propose that stimulation with current ChR2 optogenetic constructs generates subthreshold activity that contributes to the initiation of movements but, in most cases, is not sufficient to evoke a motor response
|Ohayon S,Grimaldi P,Schweers N,Tsao DY||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:16684)||2013|
Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon).
A10523 was used in immunohistochemistry - paraffin section to discuss expression of olfactomedin-like domain family members in different mammals
|Pérez-Ibave DC,González-Alvarez R,de La Luz Martinez-Fierro M,Ruiz-Ayma G,Luna-Muñoz M,Martínez-De-Villarreal LE,De Lourdes Garza-Rodríguez M,Reséndez-Pérez D,Mohamed-Noriega J,Garza-Guajardo R,Bautista-De-Lucío VM,Mohamed-Noriega K,Barboza-Quintana O,Arámburo-De-La-Hoz C,Barrera-Saldaña HA,Rodríguez-Sánchez IP||Biological research (49:null)||2016|
p53-Induced inflammation exacerbates cardiac dysfunction during pressure overload.
A10523 was used in immunohistochemistry - paraffin section to study the contribution of p53-induced inflammation to heart failure
|Yoshida Y,Shimizu I,Katsuumi G,Jiao S,Suda M,Hayashi Y,Minamino T||Journal of molecular and cellular cardiology (85:183)||2015|
Macrophage migration inhibitory factor is a possible candidate for the induction of microalbuminuria in diabetic db/db mice.
A10523 was used in immunohistochemistry - paraffin section to identify genes involved in diabetic microalbuminuria
|Watanabe T,Tomioka NH,Doshi M,Watanabe S,Tsuchiya M,Hosoyamada M||Biological and pharmaceutical bulletin (36:741)||2013|
Identified Serotonin-Releasing Neurons Induce Behavioral Quiescence and Suppress Mating in Drosophila.
A10523 was used in immunohistochemistry - free floating to study factors that regulate arousal negatively and induce states of quiescence using Drosophila
|Pooryasin A,Fiala A||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:12792)||2015|
|Not Applicable||Not Cited||
Native elongating transcript sequencing reveals human transcriptional activity at nucleotide resolution.
A10523 was used in western blot to use native elongating transcript sequencing in human cells to globally map strand-specific RNA polymerase II density at nucleotide resolution
|Mayer A,di Iulio J,Maleri S,Eser U,Vierstra J,Reynolds A,Sandstrom R,Stamatoyannopoulos JA,Churchman LS||Cell (161:541)||2015|