HUVE cells were fixed, permeabilized, and blocked using the Image-iT® Fixation/Permeabilization Kit (R37602). ZO-1 was labeled using a rabbit anti-ZO-1 antibody followed by detection with ReadyProbes™ Alexa Fluor® 488 Donkey anti-Rabbit IgG Antibody (R37118). Nuclei were stained with NucBlue® Fixed Cell ReadyProbes™ Reagent (R37606) and actin was visualized with ActinRed™ 555 ReadyProbes™ Reagent(R37112).
|Tested species reactivity||Rabbit|
|Host / Isotype||Donkey / IgG|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS with 0.1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.