Immunofluorescent analysis of Rac1 (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Rac1 (Product#PA1-091), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant Full-length Human Protein|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
PA1-091X has been successfully used in Western blot, IHC (P) and immunofluorescence applications on human and mouse samples.
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.
PA1-091X was used in western blot to elucidate the mechanism by which intronic hexanucleotide expansions in C9ORF72 contribute to amyotrophic lateral sclerosis
|Sivadasan R,Hornburg D,Drepper C,Frank N,Jablonka S,Hansel A,Lojewski X,Sterneckert J,Hermann A,Shaw PJ,Ince PG,Mann M,Meissner F,Sendtner M||Nature neuroscience (19:1610)||2016|
The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor.
PA1-091X was used in western blot to ascertain the mechanism of action of the Rb9 peptide
|Girola N,Matsuo AL,Figueiredo CR,Massaoka MH,Farias CF,Arruda DC,Azevedo RA,Monteiro HP,Resende-Lara PT,Cunha RL,Polonelli L,Travassos LR||Peptides (85:1)||2016|
The NarE protein of Neisseria gonorrhoeae catalyzes ADP-ribosylation of several ADP-ribose acceptors despite an N-terminal deletion.
PA1-091X was used in western blot to determine the enzymatic activity of NarE of Neisseria gonorrhoeae
|Rodas PI,Álamos-Musre AS,Álvarez FP,Escobar A,Tapia CV,Osorio E,Otero C,Calderón IL,Fuentes JA,Gil F,Paredes-Sabja D,Christodoulides M||FEMS microbiology letters (363:null)||2016|