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|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 350|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse IgG, mouse serum and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 8 publications below|
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Altered firing rates and patterns in interneurons in experimental cortical dysplasia.||Zhou FW,Roper SN||Cerebral cortex (New York, N.Y. : 1991) (21:1645)||2011|
|Not Applicable||Not Cited||Activation of cytosolic phospholipase A2 downstream of the Src-phospholipase D1 (PLD1)-protein kinase C γ (PKCγ) signaling axis is required for hypoxia-induced pathological retinal angiogenesis.||Zhang Q,Wang D,Singh NK,Kundumani-Sridharan V,Gadiparthi L,Rao ChM,Rao GN||The Journal of biological chemistry (286:22489)||2011|
|Not Applicable||Not Cited||PLD1-dependent PKCgamma activation downstream to Src is essential for the development of pathologic retinal neovascularization.||Zhang Q,Wang D,Kundumani-Sridharan V,Gadiparthi L,Johnson DA,Tigyi GJ,Rao GN||Blood (116:1377)||2010|
|Not Applicable||Not Cited||Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells.||Zheng D,Ezzeddine N,Chen CY,Zhu W,He X,Shyu AB||The Journal of cell biology (182:89)||2008|
|Not Applicable||Not Cited||Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.||Lloyd CM,Phillips AR,Cooper GJ,Dunbar PR||Journal of immunological methods (334:70)||2008|
|Not Applicable||Not Cited||Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1.||Zemskov EA,Mikhailenko I,Strickland DK,Belkin AM||Journal of cell science (120:3188)||2007|
|Not Applicable||Not Cited||Cellular changes in normal blood capillaries undergoing regression after inhibition of VEGF signaling.||Baffert F,Le T,Sennino B,Thurston G,Kuo CJ,Hu-Lowe D,McDonald DM||American journal of physiology. Heart and circulatory physiology (290:H547)||2006|
|Not Applicable||Not Cited||Laminin-1 redistributes postsynaptic proteins and requires rapsyn, tyrosine phosphorylation, and Src and Fyn to stably cluster acetylcholine receptors.||Marangi PA,Wieland ST,Fuhrer C||The Journal of cell biology (157:883)||2002|