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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Rho Pan Antibodies

          AbboMax

          Rho Polyclonal Antibody

          View all (8) Rho Pan antibodies

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          Cite Rho Polyclonal Antibody

          • Antibody Testing Data (1)
          Rho Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Rho Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Rho Antibody (500-10054) in WB

          Western Blot: The cell lysate derived from MCF-7 was immunoprobed by Rabbit anti-Rho (Cat#500-10054) at 1:500. An immunoreactive band is observed around ~24 kDa (2). This band is abolished by pre-incubation with immunizing peptide (1). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Rho Antibody in Western Blot (WB)
          Rho Polyclonal Antibody

          Product Details

          500-10054

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthetic peptide corresponding to the C-terminus human Rho protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with proprietary stabilizer

          Contains

          0.01% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Positive control: MCF7 Cell Lysate

          Cellular location: Cell membrane, Cytoplasm.

          Target Information

          This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Aplysia ras-related homolog 12; Aplysia RAS-related homolog 6; aplysia ras-related homolog 9 (RhoC); aplysia ras-related homolog A; aplysia ras-related homolog A1; aplysia ras-related homolog A2; aplysia ras-related homolog B; epididymis secretory protein Li 42; h12; h6; h9; induced in fatty liver dystrophy 1; oncogene RHO H12; oncogene RHO H6; oncogene RHO H9; plysia ras-related homolog A2; Ras family member A; ras homolog 9 (RhoC); ras homolog A1; ras homolog A2; ras homolog B; ras homolog family member F (in filopodia); ras homolog gene family, member A; ras homolog gene family, member A1; ras homolog gene family, member A2; ras homolog gene family, member AB; ras homolog gene family, member B; ras homolog gene family, member C; ras homolog gene family, member F (in filopodia); ras homolog gene family, member Q; Ras-like protein family member 7A; Ras-like protein TC10; RAS-like, family 7, member A; ras-related GTP-binding protein TC10; RAS-related homolog 9; Rho cDNA clone 12; Rho cDNA clone 6; Rho cDNA clone 9; rho family; Rho family GTPase; Rho family GTPase Rif; Rho in filopodia; Rho-related GTP-binding protein RhoB; Rho-related GTP-binding protein RhoC; Rho-related GTP-binding protein RhoF; Rho-related GTP-binding protein RhoQ; rhoB; rhoC GTPase; SIG-61; Silica-induced gene 61 protein; small GTP binding protein RhoA; small GTP binding protein RhoC; small GTPase Tc10; Transforming protein RhoA

          View more View less

          Gene Aliases: AA017882; AI324259; AI845056; ARH12; ARH6; ARH9; ARHA; Arha1; Arha2; ARHB; ARHC; ARHF; ARHQ; AV026554; H9; HEL-S-42; Ifld1; MST081; MSTP081; RASL7A; RHO12; RHOA; RHOB; RHOC; RHOF; RHOH12; RHOH6; RHOH9; RHOQ; RIF; TC10; TC10A

          View more View less

          UniProt ID: (Human) P61586, (Human) P62745, (Human) P08134, (Human) Q9HBH0, (Human) P17081, (Mouse) Q9QUI0, (Rat) P61589, (Mouse) P62746, (Rat) P62747, (Mouse) Q62159, (Mouse) Q8BYP3, (Mouse) Q8R527, (Rat) Q9JJL4

          View more View less

          Entrez Gene ID: (Human) 387, (Human) 388, (Human) 389, (Human) 54509, (Human) 23433, (Mouse) 11848, (Rat) 117273, (Mouse) 11852, (Rat) 64373, (Mouse) 11853, (Rat) 295342, (Rat) 690130, (Mouse) 23912, (Mouse) 104215, (Rat) 85428

          View more View less

          Function(s)
          GTPase activity protein binding GTP binding myosin binding GDP binding signal transducer activity profilin binding GBD domain binding nucleotide binding protein domain specific binding Rho GDP-dissociation inhibitor binding small GTPase
          Process(es)
          transforming growth factor beta receptor signaling pathway Rho protein signal transduction viral process cell migration substantia nigra development actin cytoskeleton organization platelet activation regulation of cell migration actin cytoskeleton reorganization positive regulation of cytokinesis regulation of actin cytoskeleton organization regulation of osteoblast proliferation Roundabout signaling pathway cleavage furrow formation apolipoprotein A-I-mediated signaling pathway positive regulation of NF-kappaB import into nucleus positive regulation of I-kappaB kinase/NF-kappaB signaling stress fiber assembly apical junction assembly endothelial cell migration ossification involved in bone maturation wound healing, spreading of cells positive regulation of neuron differentiation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling negative regulation of axonogenesis positive regulation of axonogenesis negative chemotaxis regulation of small GTPase mediated signal transduction positive regulation of stress fiber assembly Wnt signaling pathway, planar cell polarity pathway positive regulation of lipase activity trabecula morphogenesis positive regulation of protein serine/threonine kinase activity negative regulation of cell migration involved in sprouting angiogenesis mitotic spindle assembly endothelial tube lumen extension skeletal muscle satellite cell migration mitotic cleavage furrow formation regulation of cell motility cytokinesis angiogenesis intracellular protein transport apoptotic process cell adhesion endosome to lysosome transport positive regulation of endothelial cell migration cell differentiation negative regulation of cell migration positive regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell cycle endothelial tube morphogenesis cellular response to hydrogen peroxide cellular response to ionizing radiation small GTPase mediated signal transduction actin filament organization insulin receptor signaling pathway regulation of cell shape cortical actin cytoskeleton organization cellular response to insulin stimulus positive regulation of transcription from RNA polymerase II promoter GTP metabolic process positive regulation of glucose import positive regulation of filopodium assembly negative regulation of establishment of protein localization to plasma membrane cell morphogenesis regulation of transcription from RNA polymerase II promoter cytoskeleton organization cell cycle cell-matrix adhesion integrin-mediated signaling pathway skeletal muscle tissue development regulation of neuron projection development cerebral cortex cell migration forebrain radial glial cell differentiation positive regulation of cell growth androgen receptor signaling pathway positive regulation of actin filament polymerization stress-activated protein kinase signaling cascade negative regulation of intracellular steroid hormone receptor signaling pathway response to drug negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of smooth muscle contraction regulation of dendrite development cell division regulation of calcium ion transport negative regulation of cell death positive regulation of podosome assembly regulation of neural precursor cell proliferation response to hypoxia regulation of actin polymerization or depolymerization metabolic process response to mechanical stimulus response to glucose positive regulation of cell migration response to amino acid response to ethanol positive regulation of translation positive regulation of cell adhesion positive regulation of vasoconstriction neuron projection morphogenesis response to glucocorticoid cellular response to progesterone stimulus transport obsolete transformed cell apoptotic process multicellular organism development protein transport signal transduction negative regulation of glucose import
          It has to be done as per old AB suggested Products section.
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