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RhoB Antibody (PA5-77865) in IHC (P)
RhoB Polyclonal Antibody detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. RhoB Polyclonal Antibody (Product # PA5-77865) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-77865
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RhoB. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.34 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 0.1M glycine, 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736522
Product Specific Information
Positive Control: U87-MG, mouse brain, rat brain
Predicted Reactivity: Xenopus laevis (90%), Dog (99%), Pig (100%), Chicken (97%), Sheep (100%), Rhesus Monkey (89%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Rho-related GTP-binding protein (RhoB) mediates apoptosis in neoplastically transformed cells after DNA damage. While not essential for development, it does affect cell adhesion and growth factor signaling in transformed cells. RhoB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a tumor supressor - deletion of RhoB causes tumor formation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aplysia RAS-related homolog 6; aplysia ras-related homolog B; h6; oncogene RHO H6; ras homolog B; ras homolog gene family, member AB; ras homolog gene family, member B; Rho; Rho cDNA clone 6; Rho-related GTP-binding protein RhoB; rhoB
Gene Aliases: AA017882; ARH6; ARHB; MST081; MSTP081; RHOB; RHOH6
UniProt ID: (Human) P62745, (Rat) P62747, (Mouse) P62746
Entrez Gene ID: (Human) 388, (Rat) 64373, (Mouse) 11852
cytokinesis
angiogenesis
intracellular protein transport
apoptotic process
cell adhesion
Rho protein signal transduction
endosome to lysosome transport
positive regulation of endothelial cell migration
cell differentiation
platelet activation
regulation of cell migration
negative regulation of cell migration
positive regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell cycle
regulation of small GTPase mediated signal transduction
endothelial tube morphogenesis
cellular response to hydrogen peroxide
cellular response to ionizing radiation
obsolete transformed cell apoptotic process
small GTPase mediated signal transduction
transport
multicellular organism development
protein transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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