Invitrogen

RhoGAP4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (11) RhoGAP4 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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RhoGAP4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RhoGAP4 in human colon, liver, lymph node and testis using RhoGAP4 Polyclonal Antibody (Product # PA5-51499) (A) shows similar protein distribution across tissues to an independent RhoGAP4 Polyclonal Antibody (B).

RhoGAP4 Antibody in Western Blot (WB) — Western blot analysis of RhoGAP4 in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12 using a RhoGAP4 Polyclonal Antibody (Product # PA5-51499).

RhoGAP4 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of RhoGAP4 demonstrates antibody specificity. Immunohistochemical analysis of RhoGAP4 using anti-RhoGAP4 Polyclonal Antibody (Product # PA5-51499), shows significant staining of RhoGAP4 in human spleen and shows minimal or weak staining in human cerebral cortex tissues. The relative expression levels of RhoGAP4 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-51499

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human RhoGAP4 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2646536

Product Specific Information

Immunogen sequence: EHNGMRGLIP HKYITLPAGT EKQVVGAGLQ TAGESGSSPE GLLASELVHR PEPCTSPEAM GPSGHRRRCL VPASPEQHVE VDKAVAQNMD SVFKELLGKT SVRQGLGPAS TTSP

Highest antigen sequence identity to the following orthologs: Mouse - 70%, Rat - 72%.

Target Information

This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: GTPase-activating protein p115; p115; RG RhoGAP4; Rho GTPase-activating protein 4; Rho-GAP hematopoietic protein C1; Rho-type GTPase-activating protein 4

Gene Aliases: A130009C12Rik; A530015A20Rik; ARHGAP4; C1; KIAA0131; mKIAA0131; p115; RGC1; RHOGAP4; SrGAP4

UniProt ID: (Human) P98171

Entrez Gene ID: (Human) 393, (Mouse) 171207, (Rat) 246249

Molecular Function: GTPase-activating protein