Osenses

Ribeye Polyclonal Antibody

View all (25) CtBP2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Ribeye Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — IHC-P on paraffin sections of rat spleen. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Product Details

OSR00339W-100UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:300-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide from the c-terminal region of human Ribeye (CTBP2, C-terminal-binding protein 2) conjugated to an immunogenic carrier protein was used as the antigen View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Storage buffer	whole serum
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Product Specific Information

Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Specificity of this antibody: CTBP2.

Target Information

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21. 3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.