Invitrogen

S100A14 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (8) S100A14 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

S100A14 Antibody in Western Blot (WB) — Western blot analysis of S100A14 in control (vector only transfected HEK293T lysate) and S100A14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a S100A14 Polyclonal Antibody (Product # PA5-55666).

S100A14 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of S100A14 in human kidney using S100A14 Polyclonal Antibody (Product # PA5-55666) shows low expression as expected.

S100A14 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of S100A14 in human cell line MCF7 using a S100A14 Polyclonal Antibody (Product # PA5-55666) shows localization to nuclear bodies, cytosol and cell junctions.

S100A14 Antibody in Relative expression — Relative expression in different tissues in IHC: Detection of differential expression levels of S100A14 demonstrates antibody specificity. Immunohistochemical analysis of S100A14 using anti-S100A14 Polyclonal Antibody (Product # PA5-55666), shows significant staining of S100A14 in human esophagus and shows minimal or weak staining in human kidney tissues. The relative expression levels of S100A14 within each tissue is shown using RNA-Seq. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (IHC)

1:2500-1:5000

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human S100A14

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2646894

Product Specific Information

Immunogen sequence: GQCRSANAED AQEFSDVERA IETLIKNFHQ YSVEGGKETL TPSELRDLVT QQLPHLMPSN CGLEEKIANL GSCNDSKLEF RSFWELIGEA

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 37%.

Target Information

S100A14 gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.