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Immunofluorescent analysis of S100A2 showing staining in the cytoplasm and nucleus of A431 cells. A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min and stained using a S100A2 polyclonal antibody (Product # PA5-31861) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 98 of Human S100A2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-Dependent|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31861 targets S100A2 in WB applications and shows reactivity with Human samples.
The PA5-31861 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 98 of Human S100A2.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
protein S100-A2; S100 calcium-binding protein A2; S100L
CAN19; S100A2; S100L