Invitrogen

S100A8 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (27) S100A8 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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S100A8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of S100A9 in HaCaT cells using a S100A9 polyclonal antibody (Product # PA5-82881). The analysis shows localization to intermediate filaments.

S100A8 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of S100A9 in human bone marrow and duodenum tissues using a S100A9 polyclonal antibody (Product # PA5-82881). Corresponding RNA-seq data are presented for the same tissues.

S100A8 Antibody in Western Blot (WB) — Western blot analysis of S100A9 by a S100A9 polyclonal antibody (Product # PA5-82881). Analysis in human cell line SK-BR-3.

S100A8 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of S100A8 demonstrates antibody specificity. Immunohistochemical analysis of S100A8 using anti-S100A8 Polyclonal Antibody (Product #PA5-82881), shows significant staining of S100A8 in bone marrow and shows minimal or weak staining in duodenum tissues. The relative expression levels of S100A8 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-82881

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:2,500-1:5,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human S100A8 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Anitgen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2790037

Product Specific Information

Immunogen sequence: LTELEKALNS IIDVYHKYSL IKGNFHAVYR DDLKKLLETE CPQYIRKKGA DVWFKELDIN TDGAVNFQEF LILVIKM

Target Information

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Innate Immune Protein Calprotectin Interacts With and Encases Biofilm Communities of Pseudomonas aeruginosa and Staphylococcus aureus.

Frontiers in cellular and infection microbiology

Baishya J,Everett JA,Chazin WJ,Rumbaugh KP,Wakeman CA

PA5-82881 was used in Immunocytochemistry-immunoflourescence to assess the impact of Calprotectin on the biofilm architecture of P. aeruginosa and S. aureus in monomicrobial and polymicrobial culture.

Tue Aug 02 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: calgranulin A; Calgranulin-A; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; Protein S100-A8; S100 A8; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); Urinary stone protein band A

Gene Aliases: 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; S100A8

UniProt ID: (Human) P05109

Entrez Gene ID: (Human) 6279

Molecular Function: calmodulin-related

Function(s)

cytokine production leukocyte migration involved in inflammatory response acute inflammatory response chronic inflammatory response positive regulation of peptide secretion autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response response to zinc ion astrocyte development peptidyl-cysteine S-nitrosylation positive regulation of cell growth neutrophil chemotaxis sequestering of zinc ion response to lipopolysaccharide chemokine production wound healing defense response to bacterium innate immune response response to ethanol positive regulation of inflammatory response defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization neutrophil aggregation positive regulation of intrinsic apoptotic signaling pathway

Process(es)

calcium ion binding protein binding microtubule binding zinc ion binding Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding

S100A8 Polyclonal Antibody

Product Details

PA5-82881

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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