Invitrogen

S100A8 Polyclonal Antibody

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S100A8 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of S100A8 in SK-Br-3 cells using a S100A8 Polyclonal antibody (Product # PA5-86063) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

S100A8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of S100A8 of paraffin-embedded Human breast cancer tissue using a S100A8 Polyclonal antibody (Product #PA5-86063). Counter stained with hematoxylin.

S100A8 Antibody in Western Blot (WB) — Western blot analysis of S100A8 in HL-60 cell lysate using a S100A8 Polyclonal antibody (Product # PA5-86063) at a dilution of 1:100.

S100A8 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of S100A8 in THP-1 cells using a S100A8 Polyclonal Antibody (Product # PA5-86063) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Details

PA5-86063

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein within human MRP8 aa 1-93 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 50% Glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2802864

Target Information

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DOK3 maintains intestinal homeostasis by suppressing JAK2/STAT3 signaling and S100a8/9 production in neutrophils.

Cell death & disease

Loh JT,Lee KG,Lee AP,Teo JKH,Lim HL,Kim SS,Tan AH,Lam KP

PA5-86063 was used in Flow Cytometry to reveal that the immune system and microbiome function in a feed-forward manner, whereby DOK3 maintains colonic neutrophils in a quiescent state to establish a gut microbiome essential for intestinal homeostasis and protection from IBD.

Sat Nov 06 00:00:00 EDT 2021

DOK3 maintains intestinal homeostasis by suppressing JAK2/STAT3 signaling and S100a8/9 production in neutrophils.

Cell death & disease

Loh JT,Lee KG,Lee AP,Teo JKH,Lim HL,Kim SS,Tan AH,Lam KP

Sat Nov 06 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: calgranulin A; Calgranulin-A; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Protein S100-A8; S100 A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); Urinary stone protein band A

Gene Aliases: 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MIF; MRP8; NIF; P8; S100A8

UniProt ID: (Human) P05109, (Rat) P50115

Entrez Gene ID: (Human) 6279, (Rat) 116547

Molecular Function: calmodulin-related