Invitrogen

SAP97 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (15) SAP97 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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SAP97 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of DLG1 was performed using neural stem cells (NSC) differentiated into neurons. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SAP97 Polyclonal Antibody (Product # PA5-18790) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32814), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane and synaptic junctional localization. Panel e represents undifferentiated NSCs. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SAP97 Antibody in Western Blot (WB) — Western Blot staining of KELLY cell lysate using Product # PA5-18790 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

SAP97 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-SAP97 Polyclonal Antibody (Product # PA5-18790), shows induced expression of the target protein in neural stem cells (NSC) differentiated into neurons as compared to undifferentiated NSCs.

Product Details

PA5-18790

Applications

Tested Dilution

Publications

Western Blot (WB)

0.3-1 µg/mL

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (NSKTRDKGEIPDD) corresponding to the internal amino acids of DLG1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10989030

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting the non-ATP-binding pocket of the MAP kinase p38γ mediates a novel mechanism of cytotoxicity in cutaneous T-cell lymphoma (CTCL).

FEBS letters

Zhang XH,Chen CH,Li H,Hsiang J,Wu X,Hu W,Horne D,Nam S,Shively J,Rosen ST

PA5-18790 was used in Immunocytochemistry to describe here for the first time a lipid-binding-domain (LBD) in p38γ mitogen-activated protein kinase (MAPK) involved in the response of T cells to a newly identified inhibitor, CSH71.

Fri Oct 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: discs large homolog 1; discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; discs, large homolog 1 (Drosophila) transcript variant 6-v1; discs, large homolog 1 (Drosophila) transcript variant 6-v2; Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; hDlg; presynaptic protein SAP97; SAP-97; Synapse-associated protein 97

Gene Aliases: dJ1061C18.1.1; DLG1; DLGH1; hdlg; SAP-97; SAP97

UniProt ID: (Human) Q12959

Entrez Gene ID: (Human) 1739

Molecular Function: scaffold/adaptor protein